Abstract | BACKGROUND: METHOD/RESULTS: Six months after surgery, 2 groups of rats were studied: sham, and infarcted rats with HF (MI/HF+, MI size: 41.1±6.3% of total left ventricular area). In the infarcted group, microscopic evaluation revealed scattered foci of fiber necrosis in combination with inflammatory cells, phagocytosis, and increased fibrous tissue. The frequency of necrotic fibers was significantly higher in the MI/HF+ group than in the sham. The MI/HF+ group had atrophy of type I, IC/IIC, and IIA fibers compared to the sham group (P<0.05). MyoD gene expression was higher in the MI/HF+ group ( sham: 1.00±0.49; MI/HF+: 2.53±0.71 arbitrary units; P<0.001). Myogenin and MRF4 gene expression was similar in both groups. Myogenin protein levels were reduced in the MI/HF+ group ( sham: 1.00±0.21; MI/HF+: 0.74±0.21 arbitrary units; P=0.026). MyoD and MRF4 protein levels, as well as the MyHC distribution, were not different between groups. The MI/HF+ group had higher TNF-α and IL-6 serum concentrations than the sham group. CONCLUSIONS:
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Authors | Paula F Martinez, Katashi Okoshi, Leonardo A M Zornoff, Robson F Carvalho, Silvio A Oliveira Junior, Aline R R Lima, Dijon H S Campos, Ricardo L Damatto, Carlos R Padovani, Celia R Nogueira, Maeli Dal Pai-Silva, Marina P Okoshi |
Journal | Medical science monitor : international medical journal of experimental and clinical research
(Med Sci Monit)
Vol. 16
Issue 12
Pg. BR374-83
(Dec 2010)
ISSN: 1643-3750 [Electronic] United States |
PMID | 21119570
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Interleukin-6
- Myogenic Regulatory Factors
- Protein Isoforms
- Tumor Necrosis Factor-alpha
- Myosin Heavy Chains
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Topics |
- Animals
- Blotting, Western
- Electrocardiography
- Heart Failure
(complications, etiology)
- Interleukin-6
(blood)
- Male
- Muscle, Skeletal
(pathology)
- Muscular Atrophy
(etiology, metabolism, pathology)
- Myocardial Infarction
(complications)
- Myogenic Regulatory Factors
(metabolism)
- Myosin Heavy Chains
(metabolism)
- Necrosis
(etiology, pathology)
- Protein Isoforms
(metabolism)
- Rats
- Rats, Wistar
- Reverse Transcriptase Polymerase Chain Reaction
- Tumor Necrosis Factor-alpha
(blood)
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