Abstract | BACKGROUND/AIM:
Gallbladder cancers are well known to frequently exhibit TP53 as well as K-ras gene mutations. This study performed a TP53 gene investigation by PCR-SSCP and direct sequencing using both bile supernatants and tissue samples from cholecystectomy specimens lacking gallbladder cancer, in order to investigate gallbladder carcinogenesis. MATERIALS AND METHODS: Eighteen out of 294 cases, mainly of cholecystitis, were extracted by screening SSCP of bile supernatants for TP53 mutations, and investigation of their tissue samples both SSCP and direct sequencing. RESULTS: Non-neoplastic mucosal samples demonstrated shifted bands in 11 cases (61%), and mutations were confirmed in 7 cases (64%). Unexpectedly, no cases showed identical point mutations in both bile and tissue samples. G:C to A:T transitions, thought to be sporadic mutations, predominated (77%). CONCLUSION:
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Authors | Nobuyuki Yanagisawa, Kazuya Yamashita, Tatsuru Kuba, Isao Okayasu |
Journal | Anticancer research
(Anticancer Res)
Vol. 30
Issue 11
Pg. 4443-9
(Nov 2010)
ISSN: 1791-7530 [Electronic] Greece |
PMID | 21115891
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA, Neoplasm
- TP53 protein, human
- Tumor Suppressor Protein p53
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Cholecystectomy
- Cholecystitis
(genetics, surgery)
- Chronic Disease
- DNA, Neoplasm
(genetics)
- Disease Progression
- Female
- Gallbladder Neoplasms
(genetics, pathology, surgery)
- Humans
- Immunoenzyme Techniques
- Male
- Middle Aged
- Point Mutation
(genetics)
- Polymerase Chain Reaction
- Polymorphism, Single-Stranded Conformational
- Tumor Suppressor Protein p53
(genetics)
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