MicroRNA-184-mediated inhibition of tumour growth in an orthotopic murine model of neuroblastoma.

Abstract

BACKGROUND:

Neuroblastoma is a paediatric cancer which originates from precursor cells of the sympathetic nervous system. Previous studies have shown that miR-184 expression has anti-proliferative effects in neuroblastoma cells grown in culture. Therefore, it was of interest to evaluate this effect in vivo.

MATERIALS AND METHODS:

Neuroblastoma cells overexpressing miR-184 were injected retroperitoneally into CB17-SCID mice and tumour burden was assessed by measuring bioluminescence. Overall survival was also evaluated.

RESULTS:

Ectopic overexpression of miR-184 in neuroblastoma cell lines is anti-proliferative. In addition, overexpression of miR-184 led to a significant reduction in tumour growth relative to negative control-treated cohorts in a xenograft model of neuroblastoma.

CONCLUSION:

This study demonstrated for the first time that miR-184 significantly reduces tumour growth and increases overall survival in an orthotopic murine model of neuroblastoma through assessment of tumour growth and moribundity relative to control miRNA-treated cohorts.

• Authors: Amanda Tivnan, Niamh H Foley, Lorraine Tracey, Andrew M Davidoff, Raymond L Stallings
• Journal: Anticancer research (Anticancer Res) Vol. 30 Issue 11 Pg. 4391-5 (Nov 2010) ISSN: 1791-7530 [Electronic] Greece
• PMID: 21115884 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• MIRN184 microRNA, mouse
• MicroRNAs
• RNA, Messenger

Topics

• Animals
• Cell Proliferation
• Disease Models, Animal
• Gene Expression Regulation, Neoplastic (physiology)
• Humans
• Mice
• Mice, SCID
• MicroRNAs (genetics)
• Neuroblastoma (genetics, pathology)
• RNA, Messenger (genetics)
• Reverse Transcriptase Polymerase Chain Reaction
• Survival Rate
• Tumor Cells, Cultured
• Xenograft Model Antitumor Assays