Bromfenvinphos - (E,Z)-O,O-diethyl-O-[1-(2,4-dichlorophenyl)-2-bromovinyl]
phosphate (BFVF) is the
insecticide elaborated in Poland, which has been used against Varroa destructor causing honey bees disease called as varroosis. The substances that are formed as a result of
bromfenvinphos synthesis are dihydro-
bromfenvinphos (O,O-diethyl O-[1-(2,4-dichlorophenyl)
vinyl] phosphate); dibromo-
bromfenvinphos (O,O-diethyl O-[1-(2,4-dichlorophenyl)-2,2-dibromovinyl]
phosphate); 2,4-dichlorophenacyl
bromide; 2,4-dichlorophenacylidene
bromide and 2,4-dichlorophenacylidyne
bromide. In this work, we evaluated the effect of these compounds on
hemolysis and
hemoglobin oxidation (met-Hb formation) in human erythrocytes. Moreover, the changes in the size (FSC-A) and the shape (SSC-A) of red blood cells were assessed using flow cytometry and phase contrast microscopy. It was proven that
bromfenvinphos at concentrations ranging from 0.5 to 250 μM during 1h incubation did not change the parameters examined in human erythrocytes. Similarly, most of
bromfenvinphos impurities did not increase
hemolysis and
methemoglobin level nor changed the size and shape of the erythrocytes. The exception was dibromo-
bromfenvinphos, which changed the FSC-A and SSC-A parameters, as well as 2,4-dichlorophenacyl
bromide which induced
hemolysis, increased the level of met-Hb and changed erythrocytes morphology.