Abstract |
Both deregulation of tumor-suppressor genes and misexpression of microRNAs ( miRNAs) have been implicated in the development of pancreatic cancer, but their relationship during this process remains less clear. Here, we report that the expression of miR-483-3p is strongly enhanced in pancreatic cancer tissues compared to side normal tissues using a miRNA-array differential analysis. Furthermore, DPC4/Smad4 is identified as a target of miR-483-3p and their expression levels are inversely correlated in human clinical specimens. Ectopic expression of miR-483-3p significantly represses DPC4/ Smad4 protein levels in pancreatic cancer cell lines, and simultaneously promotes cell proliferation and colony formation in vitro. Our findings identify miR-483-3p as a potent regulator of DPC4/Smad4, which may provide a novel therapeutic strategy for the treatment of DPC4/Smad4-driven pancreatic cancer.
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Authors | Jun Hao, Shuyu Zhang, Yingqi Zhou, Xiangui Hu, Chenghao Shao |
Journal | FEBS letters
(FEBS Lett)
Vol. 585
Issue 1
Pg. 207-13
(Jan 03 2011)
ISSN: 1873-3468 [Electronic] England |
PMID | 21112326
(Publication Type: Journal Article)
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Copyright | Copyright © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
Chemical References |
- 3' Untranslated Regions
- MIRN483 microRNA, human
- MicroRNAs
- SMAD4 protein, human
- Smad4 Protein
- Luciferases
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Topics |
- 3' Untranslated Regions
(genetics)
- Binding Sites
(genetics)
- Blotting, Western
- Cell Line, Tumor
- Cell Proliferation
- Gene Expression Profiling
- Gene Expression Regulation, Neoplastic
- Humans
- Luciferases
(genetics, metabolism)
- MicroRNAs
(genetics)
- Mutation
- Pancreatic Neoplasms
(genetics, metabolism, pathology)
- Reverse Transcriptase Polymerase Chain Reaction
- Smad4 Protein
(genetics)
- Transfection
- Tumor Stem Cell Assay
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