Characterization of the brain proteome of rats with diabetes mellitus through two-dimensional electrophoresis and mass spectrometry.

Abstract

An understanding of the diabetes-related changes in brain proteome composition in diabetes and treatment with Cynodon dactylon may provide insights to understand the brain function associated diabetes and metabolic protein mechanism responsible for this disease and in order to discover novel preventive and therapeutic drugs. We have performed a comprehensive proteomic analysis for comparison of rat brain proteome by using advance 2-dimensional electrophoresis (2DE) combined with mass spectrometry (MALDI-TOF-MS). We report here a comparison of alloxan-induced type 1 diabetic rats and C. dactylon-treated diabetic rats at 2 weeks following diabetes onset. As a result, we identified two differentially expressed proteins from rat brain. The identified proteins were functionally classified into two groups: (i) metabolic signalling protein (PPP1R14D) and ii) vesicle transport signalling protein (RAB18). This study provides a preliminary reference map of normal rat brain that will form a basis for comparative studies on normal and pathological conditions of the brain connected with diabetes and may serve as a potential tool for clinical diagnosis, therapeutics and prognosis and may provide new insights into novel mechanisms and therapeutic targets for diabetes-associated neurological disorder.

• Authors: D Karthik, S Ravikumar
• Journal: Brain research (Brain Res) Vol. 1371 Pg. 171-9 (Jan 31 2011) ISSN: 1872-6240 [Electronic] Netherlands
• PMID: 21112318 (Publication Type: Journal Article)
• Copyright: Copyright © 2010 Elsevier B.V. All rights reserved.

Chemical References

• Hypoglycemic Agents
• Nerve Tissue Proteins
• PPP1R14D protein, rat
• Plant Extracts
• Proteome
• Solvents
• Ethanol
• Protein Phosphatase 1
• Rab18 protein, rat
• rab GTP-Binding Proteins

Topics

• Animals
• Brain Chemistry
• Cynodon (chemistry)
• Diabetes Mellitus, Experimental (drug therapy, metabolism)
• Electrophoresis, Gel, Two-Dimensional
• Ethanol
• Hypoglycemic Agents (isolation & purification, therapeutic use)
• Models, Biological
• Nerve Tissue Proteins (analysis, biosynthesis, genetics)
• Phylogeny
• Phytotherapy
• Plant Extracts (isolation & purification, therapeutic use)
• Plant Leaves (chemistry)
• Protein Phosphatase 1 (antagonists & inhibitors, biosynthesis, genetics)
• Proteome
• Rats
• Sequence Homology, Amino Acid
• Solvents
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• rab GTP-Binding Proteins (biosynthesis, genetics)