Fluoro jade-C staining in the assessment of brain injury after deep hypothermia circulatory arrest.

Abstract

OBJECTIVE:

To evaluate the efficacy of Fluoro Jade-C staining (FJC) in the assessment of brain injury after deep hypothermia circulatory arrest (DHCA).

METHODS:

Six healthy adult miniature male pigs underwent DHCA, the rectal temperature was down to 18°C, circulation was stopped , circulatory arrest was maintained for 60 minutes. On postoperative day 1, perfusion-fixation was performed on brain tissue. Cerebral cortex, hippocampus, cerebellum were taken for sampling. FJC, hematoxylin-eosin staining (HE), nissl staining (NISSL), terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) were performed to detect the histological and pathological changes. Histological scores of all slices were ranked. Comparison between the FJC and other techniques was done by analysis of variance (ANOVA) according to histological scores.

RESULTS:

All animals survived the operation. On the cerebral cortex, in comparison of FJC between HE, NISSL and TUNEL, the p value was 0.90, 0.40, 0.16 respectively (p>0.05). On the hippocampus, the comparison of FJC with HE, NISSL and TUNEL had a p value of 0.12, 0.23, 0.62 respectively (p>0.05). On the cerebellum, in comparing FJC with HE, NISSL and TUNEL, the p value was 0.96, 0.77, 0.96 respectively (p>0.05). On representative regions, the results of FJC were in accordance with that of TUNEL, NISSL and HE. Furthermore, ascertainment of brain injury is easier with FJC.

CONCLUSION:

FJC is a reliable and convenient method to assess brain injury after DHCA.

• Authors: Ren Wang, Wei-Guo Ma, Guo-Dong Gao, Qun-Xia Mao, Jun Zheng, Li-Zhong Sun, Ying-Long Liu
• Journal: Brain research (Brain Res) Vol. 1372 Pg. 127-32 (Feb 04 2011) ISSN: 1872-6240 [Electronic] Netherlands
• PMID: 21111715 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2010 Elsevier B.V. All rights reserved.

Chemical References

• Fluoresceins
• Organic Chemicals
• fluoro jade

Topics

• Animals
• Brain (pathology)
• Brain Injuries (diagnosis, etiology, pathology)
• Cardiopulmonary Bypass (methods)
• Circulatory Arrest, Deep Hypothermia Induced (adverse effects)
• Disease Models, Animal
• Fluoresceins
• In Situ Nick-End Labeling (methods)
• Male
• Neurons (metabolism, pathology)
• Organic Chemicals
• Swine