Abstract | BACKGROUND: METHODS: The airway levels and localization of bFGF, FGF receptor-1 and TGF-β1 were determined by ELISA, immunohistology and image analysis in the remodeled airways of chronic OVA-challenged mice treated with either corticosteroids or diluent. In vitro cultures of bone narrow-derived macrophages were used to determine whether bFGF induced TGF-β1 expression. RESULTS: Mice chronically challenged with OVA developed significant airway remodeling that was associated with significantly increased levels of bFGF and TGF-β1. Immunohistochemistry demonstrated significantly increased bFGF and FGF receptor-1 expression by peri- bronchial F4/80+ cells. Double-label immunofluorescence microscopy studies demonstrated that peribronchial macrophages coexpressed bFGF and TGF-β1. In vitro studies demonstrated that incubation of bone marrow-derived macrophages with bFGF induced expression of TGF-β1. Mice treated with corticosteroids and subjected to chronic OVA challenge had significantly reduced levels of bFGF, FGF receptor-1, peribronchial TGF-β1+ cells and airway remodeling. CONCLUSIONS: Overall, this study demonstrates that allergen challenge stimulates peribronchial macrophages to coexpress bFGF and TGF-β1 and that bFGF may potentiate macrophage release of TGF-β1 through autocrine and/or paracrine pathways.
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Authors | Hye Young Yum, Jae Youn Cho, Marina Miller, David H Broide |
Journal | International archives of allergy and immunology
(Int Arch Allergy Immunol)
Vol. 155
Issue 1
Pg. 12-22
( 2011)
ISSN: 1423-0097 [Electronic] Switzerland |
PMID | 21109744
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Copyright | Copyright © 2010 S. Karger AG, Basel. |
Chemical References |
- Adrenal Cortex Hormones
- Allergens
- Antigens, Differentiation
- Transforming Growth Factor beta1
- monocyte-macrophage differentiation antigen
- Fibroblast Growth Factor 2
- Ovalbumin
- Fgfr1 protein, mouse
- Receptor, Fibroblast Growth Factor, Type 1
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Topics |
- Adrenal Cortex Hormones
(pharmacology, therapeutic use)
- Airway Remodeling
(immunology)
- Allergens
(immunology)
- Animals
- Antigens, Differentiation
(metabolism)
- Asthma
(drug therapy, immunology, metabolism, pathology)
- Bronchoalveolar Lavage Fluid
(chemistry, cytology)
- Cell Count
- Disease Models, Animal
- Eosinophils
(pathology)
- Epithelial Cells
(drug effects, metabolism)
- Female
- Fibroblast Growth Factor 2
(metabolism, pharmacology)
- Leukocytes, Mononuclear
(immunology, metabolism)
- Lung
(drug effects, metabolism, pathology)
- Macrophages
(drug effects, immunology, metabolism)
- Male
- Mice
- Mice, Inbred BALB C
- Ovalbumin
(administration & dosage, immunology)
- Receptor, Fibroblast Growth Factor, Type 1
(metabolism)
- Transforming Growth Factor beta1
(metabolism)
- Vaccination
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