Acupuncture at
acupoints GB34 and LR3 has been reported to inhibit nigrostriatal degeneration in
Parkinsonism models, yet the genes related to this preventive effect of acupuncture on the nigrostriatal dopaminergic system remain elusive. This study investigated gene expression profile changes in the striatal region of
1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (
MPTP)-induced Parkinsonism models after acupuncture at the
acupoints GB34 and LR3 using a whole transcript genechip microarray (Affymetrix genechip mouse gene 1.0 ST array). It was confirmed that acupuncture at these
acupoints could inhibit the decrease of
tyrosine hydroxylase and
dopamine transporter in the nigrostriatal region of the
MPTP model while acupuncture at the non-
acupoints could not counteract this decrease. Genechip gene array analysis (fold change cutoff 1.3 and P < 0.05) showed that 12 of the 69 probes up-regulated in
MPTP when compared to the control were down-regulated by acupuncture at the
acupoints. Of these 12 probes, 11 probes (nine annotated genes) were exclusively down-regulated by acupuncture only at the
acupoints; the Gfral gene was excluded because it was commonly down-regulated by acupuncture at both the
acupoints and the non-
acupoints. In addition, 28 of the 189 probes down-regulated in
MPTP when compared to the control were up-regulated by acupuncture at the
acupoints. Of these 28 probes, 19 probes (seven annotated genes) were exclusively up-regulated by acupuncture only at the
acupoints while nine probes were commonly up-regulated by acupuncture at both the
acupoints and the non-
acupoints. The regulation patterns of representative genes in real-time RT-PCR correlated with those of the genes in the microarray. These results suggest that the 30 probes (16 annotated genes), which are affected by
MPTP and acupuncture only at the
acupoints, are responsible for exerting in the striatal regions the inhibitory effect of acupuncture at the
acupoints on
MPTP-induced striatal degeneration.