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Overexpression of AtFRO6 in transgenic tobacco enhances ferric chelate reductase activity in leaves and increases tolerance to iron-deficiency chlorosis.

Abstract
The Arabidopsis gene FRO6(AtFRO6) encodes ferric chelate reductase and highly expressed in green tissues of plants. We have expressed the gene AtFRO6 under the control of a 35S promoter in transgenic tobacco plants. High-level expression of AtFRO6 in transgenic plants was confirmed by northern blot analysis. Ferric reductase activity in leaves of transgenic plants grown under iron-sufficient or iron-deficient conditions is 2.13 and 1.26 fold higher than in control plants respectively. The enhanced ferric reductase activity led to increased concentrations of ferrous iron and chlorophyll, and reduced the iron deficiency chlorosis in the transgenic plants, compared to the control plants. In roots, the concentration of ferrous iron and ferric reductase activity were not significantly different in the transgenic plants compared to the control plants. These results suggest that FRO6 functions as a ferric chelate reductase for iron uptake by leaf cells, and overexpression of AtFRO6 in transgenic plants can reduce iron deficiency chlorosis.
AuthorsLi-Ya Li, Qiu-Yi Cai, Dian-Si Yu, Chang-Hong Guo
JournalMolecular biology reports (Mol Biol Rep) Vol. 38 Issue 6 Pg. 3605-13 (Aug 2011) ISSN: 1573-4978 [Electronic] Netherlands
PMID21104018 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Chlorophyll
  • Iron
  • FMN Reductase
  • ferric citrate iron reductase
Topics
  • Adaptation, Physiological
  • Arabidopsis (enzymology)
  • Blotting, Northern
  • Blotting, Southern
  • Chlorophyll (metabolism)
  • FMN Reductase (genetics, metabolism)
  • Genetic Vectors (genetics)
  • Iron (metabolism)
  • Iron Deficiencies
  • Plant Diseases (genetics)
  • Plant Leaves (enzymology)
  • Plants, Genetically Modified
  • Tobacco (genetics)
  • Transformation, Genetic

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