Photosensitizer loaded HSA nanoparticles II: in vitro investigations.

Abstract

The photosensitizing efficiency of human serum albumin (HSA) nanoparticles loaded with the photosensitizers meta-tetra(hydroxy-phenyl)-chlorin (mTHPC) and meta-tetra(hydroxy-phenyl)-porphyrin (mTHPP) was investigated in vitro. The endocytotic intracellular uptake, and the time dependent drug release caused by nanoparticle decomposition of the PS loaded HSA nanoparticles were studied on Jurkat cells in suspension. The photoxicity as well as the intracellular singlet oxygen ((1)O(2)) generation were investigated in dependence on the incubation time. The obtained results show that HSA nanoparticles are promising carriers for the clinical used mTHPC (Foscan). After release the ((1)O(2)) generation as well as the phototoxicity are more efficient compared with mTHPC applied without the HSA nanoparticles.

• Authors: Annegret Preuss, Kuan Chen, Steffen Hackbarth, Matthias Wacker, Klaus Langer, Beate Röder
• Journal: International journal of pharmaceutics (Int J Pharm) Vol. 404 Issue 1-2 Pg. 308-16 (Feb 14 2011) ISSN: 1873-3476 [Electronic] Netherlands
• PMID: 21094228 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2010 Elsevier B.V. All rights reserved.

Chemical References

• Drug Carriers
• Mesoporphyrins
• Photosensitizing Agents
• Porphyrins
• Serum Albumin
• tetra(3-hydroxyphenyl)porphine
• Singlet Oxygen
• temoporfin

Topics

• Chemistry, Pharmaceutical
• Drug Carriers
• Drug Compounding
• Endocytosis
• Humans
• Jurkat Cells
• Kinetics
• Mesoporphyrins (chemistry, metabolism, pharmacology)
• Microscopy, Confocal
• Nanoparticles
• Nanotechnology
• Photosensitizing Agents (chemistry)
• Porphyrins (chemistry, metabolism, pharmacology)
• Serum Albumin (chemistry)
• Singlet Oxygen (metabolism)
• Solubility
• Technology, Pharmaceutical (methods)