Abstract | INTRODUCTION: The purpose of this in vitro study was to investigate the migration of dental pulp stem cells (DPSCs) in response to chemotactants and extracellular matrix proteins (EMPs). This DPSC signaling information is needed to help understand tooth regeneration after injury and to develop some future regenerative endodontic therapies. METHODS: RESULTS: S1P induced more vigorous DPSC migration in comparison with the other TGF- β1, FGF, or EFG chemotactants (ANOVA, P < .05). Laminin induced more vigorous DPSC migration in comparison with the other EMPs (ANOVA, P < .05). CONCLUSIONS: The EMPs, particularly laminin, and chemotactants, particularly S1P and TGF-β1, were found to be important promoters of DPSC migration. The interplay between the EMPs, blood lipid, serum, and chemotactants suggests that the migration of DPSC is highly regulated. Specific chemotactants and EMPs might mediate the process of pulp-dentin regeneration after tooth injury, and they could be used as part of regenerative endodontic therapy.
|
Authors | Cameron Howard, Peter E Murray, Kenneth N Namerow |
Journal | Journal of endodontics
(J Endod)
Vol. 36
Issue 12
Pg. 1963-6
(Dec 2010)
ISSN: 1878-3554 [Electronic] United States |
PMID | 21092813
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2010 American Association of Endodontists. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Chemotactic Factors
- Extracellular Matrix Proteins
- Laminin
- Lysophospholipids
- Recombinant Proteins
- Transforming Growth Factor beta1
- sphingosine 1-phosphate
- Sphingosine
|
Topics |
- Adult Stem Cells
(drug effects, physiology)
- Analysis of Variance
- Cell Culture Techniques
- Cell Line
- Chemotactic Factors
(pharmacology)
- Chemotaxis
(drug effects, physiology)
- Dental Pulp
(cytology, drug effects)
- Endothelial Cells
(drug effects)
- Extracellular Matrix Proteins
(pharmacology)
- Humans
- Laminin
(pharmacology)
- Lysophospholipids
(pharmacology)
- Myocytes, Smooth Muscle
(drug effects)
- Recombinant Proteins
(pharmacology)
- Regeneration
(drug effects)
- Signal Transduction
(drug effects)
- Sphingosine
(analogs & derivatives, pharmacology)
- Transforming Growth Factor beta1
(pharmacology)
|