HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Phospholipase C beta 1 expression in the dorsolateral prefrontal cortex from patients with schizophrenia at different stages of illness.

AbstractOBJECTIVE:
Our recent microarray study detected decreases in the expression of phospholipase C beta 1 mRNA in the dorsolateral prefrontal cortex from subjects with schizophrenia at different stages of illness. Thus we aimed to validate and extend these findings.
METHOD:
We measured levels of mRNA and protein for phospholipase C beta 1 variant a and b using real-time PCR and western blot analysis, respectively, in the dorsolateral prefrontal cortex from subjects with schizophrenia, who had a short (< 7 years) or long (> 22 years) duration of illness.
RESULTS:
Compared to age/sex matched controls, levels of phospholipase C beta 1 variant a and b mRNAs were decreased (-33% and -50%, respectively) in short duration schizophrenia. By contrast, only variant a mRNA was decreased (-24%) in long duration schizophrenia. There was no significant difference in the protein levels of either phospholipase C beta 1 variant in schizophrenia, irrespective of duration of illness (variant a; P = 0.84, variant b; P = 0.73).
CONCLUSION:
Our data confirm that phospholipase C beta 1 transcript levels are decreased in the dorsolateral prefrontal cortex from subjects with schizophrenia. However, the changes in levels of mRNA do not translate into a change at the level of protein. It is possible protein expression is regulated independently of mRNA and it remains to be determined whether there is a functional consequence of this change in mRNA relating to the pathophysiology of schizophrenia.
AuthorsMadhara Udawela, Elizabeth Scarr, Anthony J Hannan, Elizabeth A Thomas, Brian Dean
JournalThe Australian and New Zealand journal of psychiatry (Aust N Z J Psychiatry) Vol. 45 Issue 2 Pg. 140-7 (Feb 2011) ISSN: 1440-1614 [Electronic] England
PMID21091263 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Validation Study)
Chemical References
  • Isoenzymes
  • PLCB1 protein, human
  • Phospholipase C beta
  • Cyclophilin A
Topics
  • Adult
  • Cyclophilin A (biosynthesis)
  • Female
  • Gene Expression Regulation (genetics)
  • Humans
  • Isoenzymes (biosynthesis)
  • Male
  • Middle Aged
  • Phospholipase C beta (biosynthesis)
  • Prefrontal Cortex (enzymology)
  • Schizophrenia (diagnosis, enzymology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: