Abstract | OBJECTIVE: Our recent microarray study detected decreases in the expression of phospholipase C beta 1 mRNA in the dorsolateral prefrontal cortex from subjects with schizophrenia at different stages of illness. Thus we aimed to validate and extend these findings. METHOD: We measured levels of mRNA and protein for phospholipase C beta 1 variant a and b using real-time PCR and western blot analysis, respectively, in the dorsolateral prefrontal cortex from subjects with schizophrenia, who had a short (< 7 years) or long (> 22 years) duration of illness. RESULTS: Compared to age/sex matched controls, levels of phospholipase C beta 1 variant a and b mRNAs were decreased (-33% and -50%, respectively) in short duration schizophrenia. By contrast, only variant a mRNA was decreased (-24%) in long duration schizophrenia. There was no significant difference in the protein levels of either phospholipase C beta 1 variant in schizophrenia, irrespective of duration of illness (variant a; P = 0.84, variant b; P = 0.73). CONCLUSION: Our data confirm that phospholipase C beta 1 transcript levels are decreased in the dorsolateral prefrontal cortex from subjects with schizophrenia. However, the changes in levels of mRNA do not translate into a change at the level of protein. It is possible protein expression is regulated independently of mRNA and it remains to be determined whether there is a functional consequence of this change in mRNA relating to the pathophysiology of schizophrenia.
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Authors | Madhara Udawela, Elizabeth Scarr, Anthony J Hannan, Elizabeth A Thomas, Brian Dean |
Journal | The Australian and New Zealand journal of psychiatry
(Aust N Z J Psychiatry)
Vol. 45
Issue 2
Pg. 140-7
(Feb 2011)
ISSN: 1440-1614 [Electronic] England |
PMID | 21091263
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Validation Study)
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Chemical References |
- Isoenzymes
- PLCB1 protein, human
- Phospholipase C beta
- Cyclophilin A
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Topics |
- Adult
- Cyclophilin A
(biosynthesis)
- Female
- Gene Expression Regulation
(genetics)
- Humans
- Isoenzymes
(biosynthesis)
- Male
- Middle Aged
- Phospholipase C beta
(biosynthesis)
- Prefrontal Cortex
(enzymology)
- Schizophrenia
(diagnosis, enzymology)
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