[Radioiodide treatment mediated by adenovirus transfer of human sodium iodide symporter gene into androgen-independent prostate cancer].

Abstract

This study sought to probe the feasibility of instituting a radioiodide treatment for androgen-independent prostate cancer by adenovirus transfer of the hNIS gene. A recombinant adenovirus, Ad-CMV-NIS, that expressed the NIS gene under the control of cytomegalovirus (CMV) promoter was constructed. In vitro, after infection with Ad-CMV-NIS,PC-3 prostate cancer cells exhibited an uptake of perchlorate-sensitive iodide, approximately 120 times higher than that exhibited by negative control Ad-CMV-GFP-infected cells. The half-time of efflux was 26.6 min. Clonogenic assays demonstrated that Ad-CMV-NIS-infected cancer cells were selectively killed by exposure to 131I. In vivo, Ad-CMV-NIS infected tumors showed significant radioiodine accumulation (16.30 +/- 8.72)% ID/g at 2h postinjection) with an effective half-life of 5.4h. The tumor could be clearly visualized by 131I scintigraphy. These data indicate that infection with Ad-CMV-NIS is an efficient way to induce radioiodide uptake in vitro and in vivo, thus suggesting that NIS-based gene therapy has the potential for use in androgen-independent prostate cancer.

• Authors: Rui Huang, Xiajuan Ma, Suping Li, Da Mu, Rixiang Gong, Anren Kuang
• Journal: Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi (Sheng Wu Yi Xue Gong Cheng Xue Za Zhi) Vol. 27 Issue 5 Pg. 1080-4 (Oct 2010) ISSN: 1001-5515 [Print] China
• PMID: 21089675 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Iodine Radioisotopes
• Symporters
• sodium-iodide symporter

Topics

• Adenoviridae (genetics, metabolism)
• Genetic Therapy (methods)
• Humans
• Iodine Radioisotopes (therapeutic use)
• Male
• Prostatic Neoplasms (genetics, metabolism, radiotherapy)
• Symporters (genetics)
• Transfection (methods)