Abstract | BACKGROUND: METHODS:
Lactate concentration was measured using a spectrophotometric method. Amino acids in serum and CSF were analyzed by ion exchange chromatography with ninhydrin detection. RESULTS: Average blood and CSF- lactate levels were significantly higher in children with mitochondrial disorders (3.87 ± 0.48 and 4.43 ± 0.55 mmol/l) and meningitis (2.77 ± 0.45 and 8.58 ± 1.08 mmol/l) than in children with epilepsy (1.72 ± 0.13 and 1.62 ± 0.04 mmol/l), psychomotor retardation (1.79 ± 1.40 and 1.68 ± 0.06 mmol/l) or meningism (1.70 ± 0.13 and 1.64 ± 0.07 mmol/l). Blood and CSF- alanine levels were also higher in children with mitochondrial disorders (558 ± 44 and 51 ± 8 μmol/l) than in children with epilepsy (327 ± 23 and 27 ± 3 μmol/l) or psychomotor retardation (323 ± 27 and 26 ± 3 μmol/l). The CSF- lactate levels of children with epilepsy were similar whether the samples were obtained 3 ± 0.6 h after an attack of brief seizures or from children without history of recent seizures. CONCLUSION: Elevated cerebrospinal fluid lactate level is a reliable marker pointing to mitochondrial origin of disease, even in children who have recently suffered short-lasting seizures. Some children with mitochondrial disorders manifest only mild or intermittent elevation of lactate levels.
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Authors | Martin Magner, Karol Szentiványi, Ivana Svandová, Pavel Ješina, Markéta Tesařová, Tomáš Honzík, Jiří Zeman |
Journal | European journal of paediatric neurology : EJPN : official journal of the European Paediatric Neurology Society
(Eur J Paediatr Neurol)
Vol. 15
Issue 2
Pg. 101-8
(Mar 2011)
ISSN: 1532-2130 [Electronic] England |
PMID | 21075023
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2010 European Paediatric Neurology Society. Published by Elsevier Ltd. All rights reserved. |
Chemical References |
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Topics |
- Adolescent
- Biomarkers
(cerebrospinal fluid)
- Brain Diseases, Metabolic
(cerebrospinal fluid, diagnosis)
- Child, Preschool
- Epilepsy
(cerebrospinal fluid, diagnosis)
- Female
- Humans
- Infant
- Infant, Newborn
- Lactic Acid
(biosynthesis, cerebrospinal fluid)
- Male
- Mitochondrial Diseases
(cerebrospinal fluid, diagnosis)
- Reproducibility of Results
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