Abstract | BACKGROUND: Common single-nucleotide polymorphisms (SNPs) in microRNAs ( miRNA) have been shown to be associated with susceptibility to several human cancers. We evaluated the associations of three SNPs (rs11614913, rs2910164, and rs3746444) in pre- miRNAs (miR-196a2, miR-146a, and miR-499) with the risk of gastric cancer (GC) and peptic ulcer diseases, and with the severity of Helicobacter pylori-induced gastritis in Japanese population. METHODS: The rs11614913 (C>T), rs2910164 (G>C), and rs3746444 (A>G) SNPs were genotyped in 552 GC, and 697 non- cancer subjects, including 141 gastric and 73 duodenal ulcer, and 483 non- ulcer subjects. The degree of histologic gastritis was classified according to the updated Sydney System, and the serum pepsinogen levels were measured in selected 579 and 204 cases. RESULTS: The rs2910164 CC genotype held a significantly higher risk of GC when compared to non- cancer subjects (adjusted odds ratio (OR) = 1.30, 95% confidence interval (CI) = 1.02-1.66, p =.03). Similarly, the rs2910164 C carrier was associated with higher risk of GC when compared to both non- cancer and non- ulcer subjects (OR = 1.39, 95%CI = 1.00-1.93, p =.05, adjusted OR = 1.57, 95%CI = 1.09-2.27, p =.016, respectively). The rs2910164 CC genotype was associated with non-cardia and upper third, diffuse type and advanced stage GC. The rs11614913 TT genotype was associated with higher degree of mononuclear cell infiltration (score 0-1 vs 2∼, adjusted OR = 1.62, 95%CI = 1.05-2.49, p =.03). CONCLUSIONS: The rs2910164 (G>C) SNP in the miR-146a is associated with susceptibility to GC. In addition, the rs11614913 (C>T) SNP in the miR-196a2 is associated with the degree of H. pylori-induced mononuclear cell infiltration.
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Authors | Masaaki Okubo, Tomomitsu Tahara, Tomoyuki Shibata, Hiromi Yamashita, Masakatsu Nakamura, Daisuke Yoshioka, Joh Yonemura, Takamitsu Ishizuka, Tomiyasu Arisawa, Ichiro Hirata |
Journal | Helicobacter
(Helicobacter)
Vol. 15
Issue 6
Pg. 524-31
(Dec 2010)
ISSN: 1523-5378 [Electronic] England |
PMID | 21073609
(Publication Type: Journal Article)
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Copyright | © 2010 Blackwell Publishing Ltd. |
Chemical References |
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Topics |
- Adult
- Aged
- Asian People
(genetics)
- Case-Control Studies
- Female
- Genetic Predisposition to Disease
- Genetic Variation
- Helicobacter Infections
(genetics, immunology, microbiology)
- Helicobacter pylori
(isolation & purification, physiology)
- Humans
- Japan
- Male
- MicroRNAs
(genetics)
- Middle Aged
- Polymorphism, Single Nucleotide
- Risk Factors
- Stomach Neoplasms
(genetics, immunology, microbiology)
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