The hepatic biosynthesis of rat thyroxine binding globulin (TBG): demonstration, ontogenesis, and up-regulation in experimental hypothyroidism.

Abstract

Using a human thyroxine binding globulin (TBG) cDNA probe, we demonstrate that rat liver contains two TBG mRNA species of different length, consisting of about 1.8 Kb and 2.4 Kb respectively. Slot blot analysis of the hepatic mRNAs from rats of different age reveals a fair correlation between the developmental trend of the messengers and that of the TBG circulating levels. Finally Northern blot and slot studies demonstrate that the increase of serum TBG induced in adults by thyroidectomy actually reflects an enhanced hepatic biosynthesis of the protein.

• Authors: R Vranckx, M Rouaze, L Savu, E A Nunez, C Beaumont, I L Flink
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 167 Issue 1 Pg. 317-22 (Feb 28 1990) ISSN: 0006-291X [Print] United States
• PMID: 2106883 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA Probes
• RNA, Messenger
• Thyroxine-Binding Proteins
• DNA

Topics

• Animals
• Blotting, Northern
• DNA (genetics)
• DNA Probes
• Humans
• Hypothyroidism (genetics, metabolism)
• Liver (metabolism)
• Nucleic Acid Hybridization
• RNA, Messenger (analysis)
• Rats
• Rats, Inbred Strains
• Thyroid Gland (physiology)
• Thyroidectomy
• Thyroxine-Binding Proteins (biosynthesis, genetics, metabolism)
• Up-Regulation