HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

β 1-4 mannobiose enhances Salmonella-killing activity and activates innate immune responses in chicken macrophages.

Abstract
Salmonella spp. is one of the major causes of food-borne illness in humans, and Salmonella enteritidis (SE) infection in commercial poultry is a world-wide problem. Here we have investigated the in vitro immune-modulating effects of β 1-4 mannobiose (MNB), which was previously found to prevent SE infection in vivo in chickens, using chicken macrophage (MQ-MCSU) cells. Treatment of MQ-NCSU cells with MNB dose-dependently increased both phagocytic activity and Salmonella-killing activity of macrophages, with the highest reduction in SE viability observed at a concentration of 40 μg/ml at 48 h post-infection. Likewise, both hydrogen peroxide (H(2)O(2)) and nitric oxide (NO) production were increased in a dose-dependent manner by MNB. Gene expression analysis of MNB-treated macrophages revealed significant increases in the expression of iNOS, NOX-1, IFN-γ, NRAMP1, and LITAF, genes critical for host defense and antimicrobial activity, when compared to untreated cells. This data confirms that MNB possesses potent innate immune-modulating activities and can up-regulate antibacterial defenses in chicken macrophages.
AuthorsMasahisa Ibuki, Jennifer Kovacs-Nolan, Kensuke Fukui, Hiroyuki Kanatani, Yoshinori Mine
JournalVeterinary immunology and immunopathology (Vet Immunol Immunopathol) Vol. 139 Issue 2-4 Pg. 289-95 (Feb 15 2011) ISSN: 1873-2534 [Electronic] Netherlands
PMID21067819 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2010 Elsevier B.V. All rights reserved.
Chemical References
  • Mannans
  • mannobiose
  • Nitric Oxide
  • Hydrogen Peroxide
Topics
  • Animals
  • Cell Line
  • Chickens
  • Dose-Response Relationship, Drug
  • Gene Expression Regulation (physiology)
  • Hydrogen Peroxide (metabolism)
  • Immunity, Innate (physiology)
  • Macrophages (drug effects, metabolism)
  • Mannans (administration & dosage, pharmacology)
  • Nitric Oxide (metabolism)
  • Phagocytosis (drug effects, physiology)
  • Salmonella enteritidis (physiology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: