Abstract |
Salmonella spp. is one of the major causes of food-borne illness in humans, and Salmonella enteritidis (SE) infection in commercial poultry is a world-wide problem. Here we have investigated the in vitro immune-modulating effects of β 1-4 mannobiose (MNB), which was previously found to prevent SE infection in vivo in chickens, using chicken macrophage (MQ-MCSU) cells. Treatment of MQ-NCSU cells with MNB dose-dependently increased both phagocytic activity and Salmonella-killing activity of macrophages, with the highest reduction in SE viability observed at a concentration of 40 μg/ml at 48 h post- infection. Likewise, both hydrogen peroxide (H(2)O(2)) and nitric oxide (NO) production were increased in a dose-dependent manner by MNB. Gene expression analysis of MNB-treated macrophages revealed significant increases in the expression of iNOS, NOX-1, IFN-γ, NRAMP1, and LITAF, genes critical for host defense and antimicrobial activity, when compared to untreated cells. This data confirms that MNB possesses potent innate immune-modulating activities and can up-regulate antibacterial defenses in chicken macrophages.
|
Authors | Masahisa Ibuki, Jennifer Kovacs-Nolan, Kensuke Fukui, Hiroyuki Kanatani, Yoshinori Mine |
Journal | Veterinary immunology and immunopathology
(Vet Immunol Immunopathol)
Vol. 139
Issue 2-4
Pg. 289-95
(Feb 15 2011)
ISSN: 1873-2534 [Electronic] Netherlands |
PMID | 21067819
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2010 Elsevier B.V. All rights reserved. |
Chemical References |
- Mannans
- mannobiose
- Nitric Oxide
- Hydrogen Peroxide
|
Topics |
- Animals
- Cell Line
- Chickens
- Dose-Response Relationship, Drug
- Gene Expression Regulation
(physiology)
- Hydrogen Peroxide
(metabolism)
- Immunity, Innate
(physiology)
- Macrophages
(drug effects, metabolism)
- Mannans
(administration & dosage, pharmacology)
- Nitric Oxide
(metabolism)
- Phagocytosis
(drug effects, physiology)
- Salmonella enteritidis
(physiology)
|