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[Protective effects of melatonin on ischemia-reperfusion injury of skeletal muscle].

AbstractOBJECTIVES:
In this study, we investigated the antioxidant protective effects of melatonin on skeletal muscles of Wistar albino-type rats with acute ischemia/reperfusion (I/R) injury.
MATERIALS AND METHODS:
Twenty-eight Wistar albino-type male rats weighing between 334 to 422 g were included in this experimental study. The rats were randomly allocated into three groups including sham, I/R and I/R + melatonin groups, respectively. Limb ischemia was achieved by clamping femoral arteries. After a two-hour ischemia period followed by 1.5-hour reperfusion, muscle samples were collected for biochemical analysis and histopathological examination.
RESULTS:
Muscle tissues of I/R groups revealed significantly higher antioxidant enzyme (superoxide dismutase, glutathione peroxidase, catalase) activities, and increased levels of malondialdehyde, nitric oxide, and protein carbonyl content compared to the control group (p<0.001). Levels of these parameters in muscle revealed significant reductions in the I/R + melatonin group compared to the I/R group (p<0.001). Histopathological examination of ischemic muscles in the I/R group showed significant degeneration and inflammation compared to the control group whereas melatonin administered ischemic muscles showed significant reduction in degeneration and inflammation compared to the I/R group (p<0.001).
CONCLUSION:
In the present skeletal muscle acute I/R injury model, protective effects of melatonin against reperfusion injury have been revealed. We suggest that the protective effect of melatonin against I/R damage in cases of extremity injuries with acute vascular compromise, extremity surgery with prolonged tourniquet time and acute compartment syndrome should be investigated with clinical trials.
AuthorsMehmet Erdem, Bora Bostan, Taner Güneş, Fatih Özkan, Cengiz Sen, Hüseyin Özyurt, Reşit Doğan Köseoğlu, Hasan Erdoğan
JournalEklem hastalıkları ve cerrahisi = Joint diseases & related surgery (Eklem Hastalik Cerrahisi) Vol. 21 Issue 3 Pg. 166-71 (Dec 2010) ISSN: 1309-0313 [Electronic] Turkey
Vernacular TitleMelatoninin iskelet kası iskemi-reperfüzyon yaralanması üzerine koruyucu etkisi.
PMID21067499 (Publication Type: English Abstract, Journal Article)
Chemical References
  • Reactive Oxygen Species
  • Melatonin
Topics
  • Animals
  • Extremities (blood supply)
  • Male
  • Melatonin (therapeutic use)
  • Muscle, Skeletal (drug effects, injuries, pathology)
  • Rats
  • Rats, Wistar
  • Reactive Oxygen Species
  • Reperfusion Injury (prevention & control)

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