Tolyl-specific IgE antibodies in workders with hypersensitivity to toluene diisocyanate.

Abstract

Incorporation of a p-tolyl (mono) isocyanate-human serum albumin (TMI-HSA) antigen conjugate into a solid phase radioimmunoassay permitted detection of tolyl-specific IgE antibodies in 3 of 4 workers with clinical hypersensitivity to toluene diisocyanate (TDI). By comparison 19 TDI-exposed, non-sensitized workers had antibody titers similar to those found in normal adults. High titers of tolyl-specific IgE antibodies were not correlated with high levels of total serum IgE. Use of the monofunctional isocyanate in place of TDI in antigen preparation prevented cross-linking of antigen protein, an effect usually associated with TDI, and also assured that tolyl groups were sterically exposed. TMI-HSA antigens may prove beneficial in serological or cutaneous evaluation of TDI-sensitized workers.

• Authors: M H Karol, H H Ioset, Y C Alarie
• Journal: American Industrial Hygiene Association journal (Am Ind Hyg Assoc J) Vol. 39 Issue 6 Pg. 454-8 (Jun 1978) ISSN: 0002-8894 [Print] United States
• PMID: 210646 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies
• Cyanates
• Haptens
• Toluene 2,4-Diisocyanate
• Immunoglobulin E

Topics

• Antibodies
• Antibody Specificity
• Cyanates (immunology)
• Environmental Exposure
• Haptens
• Humans
• Hypersensitivity (etiology, immunology)
• Immunoglobulin E
• Occupational Diseases (chemically induced)
• Radioimmunoassay (methods)
• Toluene 2,4-Diisocyanate (immunology)