Abstract |
The authors provide an update on a previously reported patient with in-transit metastatic melanoma of the scalp treated with topical diphencyprone (DPCP). Molecular studies implicate the thymus-derived TH17 lymphocyte subset in a remarkable immunotherapeutic regression. The authors performed RT-PCR of total RNA from paraffin-embedded tissue before and after treatment with DPCP. Before treatment with DPCP, the authors found elevated expression of IL 17C/D/E/F; after treatment there was no detectable expression. Conversely, increased expression of PLZF/CD27 and CTLA4 was seen after treatment with no expression before treatment. No expression of IL17A/B, CD7, RORgTand FoxP3 were before or after treatment. Conclusions are limited to only the time samples were obtained. Remarkable regression of an in-transit metastatic melanoma treated with the immunomodulatory agent DPCP showed gain and loss of gene expression of the TH17 pathway. Further study of this pathway from NK to NK-T to TH7 and TH1 cells both with and without accessory or dendritic cells will improve understanding of contact sensitizers as topical immunomodulators.
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Authors | Frank Martiniuk, Diona L Damian, John F Thompson, Richard A Scolyer, Kam-Meng Tchou-Wong, William R Levis |
Journal | Journal of drugs in dermatology : JDD
(J Drugs Dermatol)
Vol. 9
Issue 11
Pg. 1368-72
(Nov 2010)
ISSN: 1545-9616 [Print] United States |
PMID | 21061759
(Publication Type: Case Reports, Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Antigens, CD
- CTLA-4 Antigen
- CTLA4 protein, human
- Cyclopropanes
- Haptens
- Interleukin-17
- Kruppel-Like Transcription Factors
- Ointments
- Promyelocytic Leukemia Zinc Finger Protein
- ZBTB16 protein, human
- diphenylcyclopropenone
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Topics |
- Administration, Topical
- Aged
- Antigens, CD
(genetics)
- CTLA-4 Antigen
- Cyclopropanes
(administration & dosage)
- Haptens
(administration & dosage)
- Humans
- Interleukin-17
(genetics, metabolism)
- Kruppel-Like Transcription Factors
(genetics)
- Male
- Melanoma
(drug therapy, genetics, immunology, secondary)
- Ointments
- Promyelocytic Leukemia Zinc Finger Protein
- Reverse Transcriptase Polymerase Chain Reaction
- Scalp
- Skin Neoplasms
(drug therapy, genetics, immunology, pathology)
- Th17 Cells
(immunology)
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