Abstract | OBJECTIVE: METHODS: Wistar rats were randomly distributed into sham group (n = 18), ischemia-reperfusion (IR) group (n = 18), IR + NaHS group (n = 18) and IR + DL- propargylglycine (PAG) group (n = 18). The hepatic IR model was established by Pringle's hepatic vascular occlusion. At each of the indicated time points (1, 3 and 6 hours after IR), the serum levels of H(2)S and the hepatic CSE activity were measured. The serum levels of inflammatory factors, including TNF-α, IL-10 were determined by ELISA methods. The expression of apoptotic protein, TNF-α, in liver tissue was tested by Western blot assay, cell apoptosis was examined by TUNEL and the histological changes were examined in each group. RESULTS: The serum levels of H(2)S and CSE activity were significantly increased in group IR compared with group sham at all indicated time points (P < 0.05). The serum level of inflammatory factors (P < 0.01) and the hepatic expression of TNF-α protein (P < 0.05) were elevated obviously in group IR than that in group sham. Administration of NaHS could reduce the production of inflammatory factors in serum (P < 0.01), inhibit hepatic protein expression of TNF-α (P < 0.05) and attenuate the liver histological scores of IR injury (P < 0.05), whereas PAG aggravated them. CONCLUSION: The endogenous CSE/H(2)S system maybe involved in the pathogenesis of hepatic IR injury, which suggests that CSE/H(2)S system can protect liver from IR injury in rats by intervening in inflammatory reaction, attenuating the injury severity and inhibiting expression of apoptotic protein TNF-α.
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Authors | Kai Kang, Hong-chi Jiang, Ming-yan Zhao, Xue-ying Sun, Shang-ha Pan |
Journal | Zhonghua wai ke za zhi [Chinese journal of surgery]
(Zhonghua Wai Ke Za Zhi)
Vol. 48
Issue 12
Pg. 924-8
(Jun 15 2010)
ISSN: 0529-5815 [Print] China |
PMID | 21055229
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Sulfides
- Tumor Necrosis Factor-alpha
- Interleukin-10
- Cystathionine gamma-Lyase
- sodium bisulfide
- Hydrogen Sulfide
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Topics |
- Animals
- Apoptosis
(drug effects)
- Cystathionine gamma-Lyase
(blood, physiology)
- Disease Models, Animal
- Hydrogen Sulfide
(blood)
- Interleukin-10
(blood)
- Liver
(blood supply, metabolism, pathology)
- Male
- Random Allocation
- Rats
- Rats, Wistar
- Reperfusion Injury
(metabolism, pathology, physiopathology, prevention & control)
- Sulfides
(pharmacology)
- Tumor Necrosis Factor-alpha
(metabolism)
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