[Protection of CSE/H2S system in hepatic ischemia reperfusion injury in rats].

Abstract

OBJECTIVE:

To study the protective function and pathophysiology of cystathionine gamma-lyase (CSE)/hydrogen sulfide (H(2)S) system in hepatic ischemia-reperfusion injury (HIRI) in rats.

METHODS:

Wistar rats were randomly distributed into sham group (n = 18), ischemia-reperfusion (IR) group (n = 18), IR + NaHS group (n = 18) and IR + DL-propargylglycine (PAG) group (n = 18). The hepatic IR model was established by Pringle's hepatic vascular occlusion. At each of the indicated time points (1, 3 and 6 hours after IR), the serum levels of H(2)S and the hepatic CSE activity were measured. The serum levels of inflammatory factors, including TNF-α, IL-10 were determined by ELISA methods. The expression of apoptotic protein, TNF-α, in liver tissue was tested by Western blot assay, cell apoptosis was examined by TUNEL and the histological changes were examined in each group.

RESULTS:

The serum levels of H(2)S and CSE activity were significantly increased in group IR compared with group sham at all indicated time points (P < 0.05). The serum level of inflammatory factors (P < 0.01) and the hepatic expression of TNF-α protein (P < 0.05) were elevated obviously in group IR than that in group sham. Administration of NaHS could reduce the production of inflammatory factors in serum (P < 0.01), inhibit hepatic protein expression of TNF-α (P < 0.05) and attenuate the liver histological scores of IR injury (P < 0.05), whereas PAG aggravated them.

CONCLUSION:

The endogenous CSE/H(2)S system maybe involved in the pathogenesis of hepatic IR injury, which suggests that CSE/H(2)S system can protect liver from IR injury in rats by intervening in inflammatory reaction, attenuating the injury severity and inhibiting expression of apoptotic protein TNF-α.

• Authors: Kai Kang, Hong-chi Jiang, Ming-yan Zhao, Xue-ying Sun, Shang-ha Pan
• Journal: Zhonghua wai ke za zhi [Chinese journal of surgery] (Zhonghua Wai Ke Za Zhi) Vol. 48 Issue 12 Pg. 924-8 (Jun 15 2010) ISSN: 0529-5815 [Print] China
• PMID: 21055229 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Sulfides
• Tumor Necrosis Factor-alpha
• Interleukin-10
• Cystathionine gamma-Lyase
• sodium bisulfide
• Hydrogen Sulfide

Topics

• Animals
• Apoptosis (drug effects)
• Cystathionine gamma-Lyase (blood, physiology)
• Disease Models, Animal
• Hydrogen Sulfide (blood)
• Interleukin-10 (blood)
• Liver (blood supply, metabolism, pathology)
• Male
• Random Allocation
• Rats
• Rats, Wistar
• Reperfusion Injury (metabolism, pathology, physiopathology, prevention & control)
• Sulfides (pharmacology)
• Tumor Necrosis Factor-alpha (metabolism)