Abstract | OBJECTIVE: METHODS: The proteins expressed were analyzed using two-dimensional gel electrophoresis. The differentially expressed proteins in tumors were also analyzed by mass spectrometry (MS). AnnexinA3 expression was examined by streptavidin peroxidase immunohistochemical technique on paraffin-embedded tissue sections from 50 patients of gallbladder cancer and 38 cases of chronic eholecystitis. RESULTS:
Protein extracts of individual sample in each type of tissues were separated on two-dimensional gels. There were forty six differentially expressed proteins in the tissue samples of gallbladder cancer. Seventeen proteins were successfully identified by MS, in which nine proteins were overexpressed in tumors and the other eight proteins were underexpressed. The positive expression rates of annexinA3 in gallbladder cancer was significantly higher than that in chronic cholecystitis, and the difference was statistically significant (74.0% vs 21.1%, P < 0.01). In the gallbladder cancer, no correlation was obtained between annexinA3 and age, gender or histologicl type (P > 0.05), but overexpression of annexinA3 correlated significantly with those cases with a lower histological grading (40.0% vs 82.5%, P < 0.05); lymph node or distant metastasis (40.9% vs 100%, P < 0.05); or a shorter survival time after operation (50.0% vs 93.8%, P < 0.05). CONCLUSIONS:
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Authors | Yi Tan, Hai-ping Meng, Qiong Wu, Fu-qiang Wang, Hao-rong Wu |
Journal | Zhonghua bing li xue za zhi = Chinese journal of pathology
(Zhonghua Bing Li Xue Za Zhi)
Vol. 39
Issue 6
Pg. 382-6
(Jun 2010)
ISSN: 0529-5807 [Print] China |
PMID | 21055154
(Publication Type: English Abstract, Journal Article)
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Chemical References |
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Topics |
- Adenocarcinoma
(metabolism, pathology, surgery)
- Adult
- Aged
- Annexin A3
(metabolism)
- Carcinoma, Adenosquamous
(metabolism, pathology, surgery)
- Carcinoma, Squamous Cell
(metabolism, pathology, surgery)
- Cholecystitis
(metabolism)
- Electrophoresis, Gel, Two-Dimensional
- Female
- Gallbladder Neoplasms
(metabolism, pathology, surgery)
- Gene Expression Profiling
- Humans
- Lymphatic Metastasis
- Male
- Middle Aged
- Neoplasm Metastasis
- Proteome
(metabolism)
- Proteomics
- Survival Rate
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