Abstract | BACKGROUND: METHODS: We investigated 2-AA for its specificity to Ps. aeruginosa and its suitability as a potential breath biomarker of colonisation or infection by Solid Phase Micro Extraction and Gas Chromatography-Mass Spectrometry (GC/MS). RESULTS: Cultures of 20 clinical strains of Ps. aeruginosa but not other respiratory pathogens had high concentrations of 2-AA in the head space of in vitro cultures when analysed by GC/MS. 2-AA was stable for 6 hours in deactivated glass sampling bulbs but was not stable in Tedlar® bags. Optimisation of GC/MS allowed detection levels of 2-AA to low pico mol/mol range in breath. The 2-AA was detected in a significantly higher proportion of subjects colonised with Ps. aeruginosa 15/16 (93.7%) than both the healthy controls 5/17 (29%) (p < 0.0002) and CF patients not colonised with Ps. aeruginosa 4/13(30.7%) (p < 0.001). The sensitivity and specificity of the 2-AA breath test compared to isolation of Ps. aeruginosa in sputum and/or BALF was 93.8% (95% CI, 67-99) and 69.2% (95% CI, 38-89) respectively. The peak integration values for 2-AA analysis in the breath samples were significantly higher in Ps. aeruginosa colonised subjects (median 242, range 0-1243) than the healthy controls (median 0, range 0-161; p < 0.001) and CF subjects not colonised with Ps. aeruginosa (median 0, range 0-287; p < 0.003). CONCLUSIONS:
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Authors | Amy J Scott-Thomas, Mona Syhre, Philip K Pattemore, Michael Epton, Richard Laing, John Pearson, Stephen T Chambers |
Journal | BMC pulmonary medicine
(BMC Pulm Med)
Vol. 10
Pg. 56
(Nov 07 2010)
ISSN: 1471-2466 [Electronic] England |
PMID | 21054900
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Acetophenones
- Biomarkers
- 2-aminoacetophenone
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Topics |
- Acetophenones
(analysis)
- Adolescent
- Adult
- Biomarkers
(analysis)
- Breath Tests
(methods)
- Child
- Cystic Fibrosis
(complications, microbiology)
- Female
- Gas Chromatography-Mass Spectrometry
- Humans
- Lung
(microbiology)
- Male
- Middle Aged
- Pseudomonas Infections
(complications, diagnosis)
- Pseudomonas aeruginosa
(chemistry, growth & development)
- Respiratory Tract Infections
(complications, diagnosis)
- Sensitivity and Specificity
- Young Adult
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