Abstract | PURPOSE: METHODS: RESULTS: Expression of MMP-3 increased and TIMP-1 decreased in the presence of 10 ng/mL TNF-α in ACBRI181 cells. Expression of MMP-1 increased and TIMP-2 decreased in the presence of 10 ng/mL TNF-α in ARPE-19 cells. These altered levels of expression were reversed by the addition of infliximab. The cell-derived proteins degraded by MMP-1 and -3 were detected in each set of cells. CONCLUSIONS: The presence of TNF-α altered expression of MMPs and TIMPs in cells comprising the BRB, and infliximab counteracted this alteration.
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Authors | Huili Li, Masahiko Yoneda, Masayuki Takeyama, Iichiro Sugita, Hinako Tsunekawa, Hiroshi Yamada, Daisuke Watanabe, Tomoyuki Mukai, Masahiro Yamamura, Masayoshi Iwaki, Masahiro Zako |
Journal | Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics
(J Ocul Pharmacol Ther)
Vol. 26
Issue 6
Pg. 549-56
(Dec 2010)
ISSN: 1557-7732 [Electronic] United States |
PMID | 21054185
(Publication Type: Journal Article)
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Chemical References |
- Anti-Inflammatory Agents
- Antibodies, Monoclonal
- RNA, Messenger
- Tissue Inhibitor of Metalloproteinase-1
- Tumor Necrosis Factor-alpha
- Tissue Inhibitor of Metalloproteinase-2
- Infliximab
- Matrix Metalloproteinases
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Topics |
- Anti-Inflammatory Agents
(pharmacology)
- Antibodies, Monoclonal
(pharmacology)
- Blood-Retinal Barrier
(drug effects, metabolism)
- Cell Line
- Electrophoresis, Polyacrylamide Gel
- Endothelial Cells
(drug effects, metabolism)
- Endothelium, Vascular
(drug effects, metabolism)
- Gene Expression Regulation
(drug effects)
- Humans
- Infliximab
- Matrix Metalloproteinases
(metabolism)
- Polymerase Chain Reaction
- RNA, Messenger
(metabolism)
- Retinal Pigment Epithelium
(drug effects, metabolism)
- Retinal Vessels
(drug effects, metabolism)
- Tissue Inhibitor of Metalloproteinase-1
(drug effects, metabolism)
- Tissue Inhibitor of Metalloproteinase-2
(drug effects, metabolism)
- Tumor Necrosis Factor-alpha
(administration & dosage, metabolism)
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