Abstract | BACKGROUND: Previously the angiotensin II receptor blocker Irbesartan has been demonstrated to reduce the risk for progression from microalbuminuria to macroalbuminuria in type 2 diabetic patients. The purpose of this study was to evaluate the effect of treatment with Irbesartan in type 2 diabetic patients with microalbuminuria on the urinary proteome. METHODS: High-resolution capillary-electrophoresis coupled to mass-spectrometry (CE-MS) was used to profile the low-molecular-weight proteome in urine of a subgroup of patients from a two year randomized irbesartan versus placebo therapy trial, which included hypertensive type 2 diabetic patients with microalbuminuria on ongoing antihypertensive medication (IRMA2-substudy). RESULTS: CONCLUSION: CE-MS analysis of urine enabled identification of peptides as potential surrogate markers for renoprotection in microalbuminuric type 2 diabetic patients, which show persistent improvement after longterm treatment with Irbesartan. The results suggest that a major benefit of treatment by Irbesartan may be improvement of collagen turnover, reduction of fibrosis. They further suggest that urinary proteome analysis could be utilized to assess potential benefit of therapeutic intervention, providing statistically significant results even on a small population.
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Authors | Sten Andersen, Harald Mischak, Petra Zürbig, Hans-Henrik Parving, Peter Rossing |
Journal | BMC nephrology
(BMC Nephrol)
Vol. 11
Pg. 29
(Nov 01 2010)
ISSN: 1471-2369 [Electronic] England |
PMID | 21040538
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Angiotensin II Type 1 Receptor Blockers
- Biphenyl Compounds
- Proteome
- Tetrazoles
- Irbesartan
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Topics |
- Albuminuria
(drug therapy, etiology, urine)
- Angiotensin II Type 1 Receptor Blockers
(therapeutic use)
- Biphenyl Compounds
(therapeutic use)
- Diabetes Mellitus, Type 2
(complications, urine)
- Diabetic Nephropathies
(etiology, prevention & control)
- Electrophoresis, Capillary
- Humans
- Irbesartan
- Mass Spectrometry
- Proteome
(analysis, drug effects, metabolism)
- Statistics, Nonparametric
- Tetrazoles
(therapeutic use)
- Urinalysis
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