Abstract | OBJECTIVE: METHODS: The isolated rat heart was perfused in a Langendorff apparatus. The effect of U50, 488H on electrical coupling parameters including onset of uncoupling, plateau time, slope and fold increase in r(t) was observed in isolated perfused rat heart subjected to global no-flow ischemia. The effect of U50, 488H on connexin 43 ( Cx43) expression of ventricular muscle during ischemia was determined by immunohistochemistry. RESULTS: CONCLUSION: These results demonstrated that U50, 488H delayed the onset of uncoupling and plateau time, decreased the maximal rate of uncoupling and increased Cx43 expression of ventricular muscle during ischemia, and these effects of U50, 488H were mediated by kappa-opioid receptor, in which activation of PKC was involved. The effect of U50, 488H on electrical coupling during ischemia was probably correlated with preservation of Cx43 in cardiac muscle.
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Authors | Hong-Jiao Mao, Bao-Ping Chen, Hui-Ping Wang, Yun-Feng Gao, Qiang Xia |
Journal | Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology
(Zhongguo Ying Yong Sheng Li Xue Za Zhi)
Vol. 26
Issue 3
Pg. 261-5
(Aug 2010)
ISSN: 1000-6834 [Print] China |
PMID | 21038665
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Benzophenanthridines
- Connexin 43
- Receptors, Opioid, kappa
- norbinaltorphimine
- Naltrexone
- 3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer
- chelerythrine
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Topics |
- 3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer
(pharmacology)
- Animals
- Benzophenanthridines
(pharmacology)
- Connexin 43
(metabolism)
- Female
- Heart
(drug effects)
- In Vitro Techniques
- Myocardial Ischemia
(metabolism, physiopathology)
- Myocardium
(metabolism)
- Naltrexone
(analogs & derivatives, pharmacology)
- Rats
- Rats, Sprague-Dawley
- Receptors, Opioid, kappa
(metabolism)
- Signal Transduction
(drug effects)
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