Staining with highly sensitive Coomassie brilliant blue SeePico™ Stain after Flamingo™ fluorescent gel stain is useful for cancer proteomic analysis by means of two-dimensional gel electrophoresis.

Abstract

Highly sensitive Coomassie brilliant blue SeePico™ Stain was applied for proteomic analysis using two-dimensional gel electrophoresis (2-DE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). After staining with Flamingo™ Fluorescent Gel Stain, the images of the protein spots were analyzed, and 424 protein spots were detected. After washing with Milli-Q water three times, the gels were re-stained with SeePico™ Stain and the images of the protein spots were analyzed; 272 spots were detected. To assess whether SeePico™ Stain alters MS analysis, a spot was picked up and was analyzed by LC-MS/MS. The MS analysis showed good protein identification. These results show a possible role for SeePico™ Stain in cancer proteomics using 2-DE and MS.

• Authors: Yasuhiro Kuramitsu, Eiko Hayashi, Futoshi Okada, Xiulian Zhang, Toshiyuki Tanaka, Yoshiya Ueyama, Kazuyuki Nakamura
• Journal: Anticancer research (Anticancer Res) Vol. 30 Issue 10 Pg. 4001-5 (Oct 2010) ISSN: 1791-7530 [Electronic] Greece
• PMID: 21036714 (Publication Type: Journal Article)

Chemical References

• Indicators and Reagents
• Neoplasm Proteins
• Proteome
• Rosaniline Dyes
• coomassie Brilliant Blue

Topics

• Amino Acid Sequence
• Animals
• Cell Line, Tumor
• Chromatography, Liquid
• Electrophoresis, Gel, Two-Dimensional (methods)
• Fibrosarcoma (chemistry, metabolism)
• Indicators and Reagents (chemistry)
• Mice
• Molecular Sequence Data
• Neoplasm Proteins (analysis, biosynthesis)
• Proteome (analysis)
• Proteomics (methods)
• Rosaniline Dyes (chemistry)
• Staining and Labeling (methods)
• Tandem Mass Spectrometry