Effects of captopril on the cysteamine-induced duodenal ulcer in the rat.

Abstract

Oxidative stress is important factor underlying in a variety of diseases. Antioxidative enzymes such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) are part of the physiological defenses against oxidative stress. Malondialdehyde (MDA) is a lipid peroxidation biomarker and its elevated level in various diseases is related to free radical damage. Cysteamine is a cytotoxic agent, acting through generation of reactive oxygen species (ROS) and may decrease defense activity of antioxidative enzymes against ROS and induce duodenal ulcer. Captopril, acts as free radical scavengers and protect against injuries from oxidative damage to tissues.The aim of this study was the evaluation of the effect of captopril against cysteamine-induced duodenal ulcer by determining duodenal damage, duodenal tissue SOD and GSH-PX activities and plasma MAD level. This study was performed on 3 groups of 7 rats each: saline, cysteamine and cysteamine plus captopril treated groups. The effect of captopril against cysteamine-induced duodenal ulcer is determined by evaluating the duodenal damage, duodenal tissue SOD and GSH-PX activities and plasma MDA level. All animals were euthanized 24h after the last treatment and 2 ml blood and duodena samples were collected for calculation of ulcer index, histopathological assessment and measurement of tissue SOD, GSH-PX activities and plasma MDA level. Cysteamine produced severe duodenal damage, decreased the activity of duodenal tissue SOD and GSH-PX and increased the plasma MDA level compared with saline pretreated rats. Pretreatment with captopril decreased the cysteamine-induced duodenal damage and plasma level of MDA and increased the activities of SOD and GSH-PX in duodenal tissue compared with cysteamine pretreated animal. Our results suggest that captopril protects against cysteamine-induced duodenal ulcer and inhibits the decrease in SOD and GSH-PX activities and lipid peroxidation by increasing antioxidant defenses.

• Authors: Firoozeh Saghaei, Iraj Karimi, Abolghasem Jouyban, Morteza Samini
• Journal: Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie (Exp Toxicol Pathol) Vol. 64 Issue 4 Pg. 373-7 (May 2012) ISSN: 1618-1433 [Electronic] Germany
• PMID: 21036019 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2010 Elsevier GmbH. All rights reserved.

Chemical References

• Biomarkers
• Free Radical Scavengers
• Radiation-Protective Agents
• Malondialdehyde
• Cysteamine
• Captopril
• Glutathione Peroxidase
• Superoxide Dismutase

Topics

• Animals
• Biomarkers (metabolism)
• Captopril (pharmacology)
• Cysteamine (toxicity)
• Disease Models, Animal
• Duodenal Ulcer (chemically induced, metabolism, pathology, prevention & control)
• Duodenum (drug effects, enzymology, pathology)
• Free Radical Scavengers (pharmacology)
• Glutathione Peroxidase (metabolism)
• Male
• Malondialdehyde (blood)
• Oxidative Stress (drug effects)
• Radiation-Protective Agents (toxicity)
• Rats
• Rats, Wistar
• Superoxide Dismutase (metabolism)