Abstract | PURPOSE: METHODS: We used a nerve conduit filled with a fibrin-based delivery system that provided controlled release of GDNF during nerve regeneration. The motor branch of the rat femoral nerve was used to assess motor nerve regeneration across a 5-mm gap. Four experimental groups (n = 4 to n = 8) were evaluated. These included GDNF with the fibrin-based delivery system ( GDNF-DS), fibrin alone, empty conduit (negative control), and nerve isograft (positive control). Nerves were harvested at 5 weeks for analysis by histomorphometry and electron microscopy. RESULTS: At 5 mm distal to the conduit or isografts, the GDNF-DS group was not significantly different from the nerve isograft group in the following histomorphometric measures: total nerve fibers, percentage of neural tissue, and nerve density. Both the GDNF-DS and isograft groups had significantly more fibers and a higher percentage of neural tissue than fibrin alone and empty conduit groups. There were no differences in fiber width among all groups. By electron microscopy, the GDNF-DS and isograft groups also demonstrated more organized nerve architecture than the fibrin alone and empty conduit groups. CONCLUSIONS: The delivery of GDNF from the fibrin-based delivery system promotes motor nerve regeneration at a level similar to an isograft in the femoral motor nerve model. This study gives insight into the potential beneficial role of GDNF in the treatment of motor nerve injuries.
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Authors | Amy M Moore, Matthew D Wood, Kristopher Chenard, Daniel A Hunter, Susan E Mackinnon, Shelly E Sakiyama-Elbert, Gregory H Borschel |
Journal | The Journal of hand surgery
(J Hand Surg Am)
Vol. 35
Issue 12
Pg. 2008-17
(Dec 2010)
ISSN: 1531-6564 [Electronic] United States |
PMID | 21035963
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2010. Published by Elsevier Inc. |
Chemical References |
- Biocompatible Materials
- Glial Cell Line-Derived Neurotrophic Factor
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Topics |
- Animals
- Biocompatible Materials
- Drug Delivery Systems
- Femoral Nerve
(anatomy & histology, physiology)
- Glial Cell Line-Derived Neurotrophic Factor
(administration & dosage, physiology)
- Male
- Nerve Regeneration
(physiology)
- Peripheral Nerve Injuries
- Rats
- Rats, Inbred Lew
- Tissue Engineering
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