Protective effect of tadalafil on ischemia/reperfusion injury of rat ovary.

Abstract

BACKGROUND/PURPOSE:

The aim of the study was to evaluate the effects of tadalafil (TDF) on ischemia/reperfusion (I/R) injury in rat ovaries.

METHODS:

Thirty-five female Sprague-Dawley rats were randomly divided into 5 groups (n = 7): sham (S), I/R1, I/R2, TDF1, and TDF2. In the I/R1 and TDF1 groups, 3-hour ischemia was followed by 12-hour reperfusion; and in the I/R2 and TDF2 groups, 3-hour ischemia was followed by 24-hour reperfusion. In the TDF groups, 30 minutes before reperfusion, a single dose of 5 mg/kg TDF was administered intraperitoneally. The ovarian tissue levels of malondialdehyde and nitric oxide (NO), and the activities of superoxide dismutase and catalase were measured biochemically. Tissue damage to ovarian tissue was scored by histopathologic examination.

RESULTS:

The tissue malondialdehyde levels were significantly higher and the catalase and superoxide dismutase activities were significantly lower in the I/R groups compared with the S and TDF groups (P < .05). The NO levels were significantly higher in the TDF1 group than the S and I/R1 groups (P < .05). Although the NO levels were increased in the TDF2 group compared with the I/R2 group, the difference was not significant. Ovarian tissue damage scores of the I/R groups were significantly higher than those of the S group (P < .05). Treatment with TDF significantly decreased the ovarian tissue damage scores in the TDF groups compared with the I/R groups (P < .05).

CONCLUSIONS:

Tadalafil is effective in preventing tissue damage induced by I/R in rat ovaries.

• Authors: Deniz Cemgil Arikan, Vedat Bakan, Ergul Belge Kurutas, Hamide Sayar, Ayhan Coskun
• Journal: Journal of pediatric surgery (J Pediatr Surg) Vol. 45 Issue 11 Pg. 2203-9 (Nov 2010) ISSN: 1531-5037 [Electronic] United States
• PMID: 21034945 (Publication Type: Comparative Study, Journal Article)
• Copyright: Copyright © 2010 Elsevier Inc. All rights reserved.

Chemical References

• Carbolines
• Phosphodiesterase 5 Inhibitors
• Nitric Oxide
• Malondialdehyde
• Tadalafil
• Catalase
• Superoxide Dismutase

Topics

• Animals
• Carbolines (administration & dosage, therapeutic use)
• Catalase (metabolism)
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Female
• Injections, Intraperitoneal
• Lipid Peroxidation (drug effects)
• Malondialdehyde (metabolism)
• Nitric Oxide (metabolism)
• Ovary (blood supply, drug effects, metabolism)
• Oxidative Stress (drug effects)
• Phosphodiesterase 5 Inhibitors (administration & dosage, therapeutic use)
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (metabolism, pathology, prevention & control)
• Spectrophotometry
• Superoxide Dismutase (metabolism)
• Tadalafil
• Treatment Outcome