N-Glycosylation of total cellular glycoproteins from the human ovarian carcinoma SKOV3 cell line and of recombinantly expressed human erythropoietin.

Abstract

Ovarian carcinoma is the leading cause of death from gynecological cancers in many Western countries. Aberrant glycosylation is an important aspect in malignant transformation and consequently in ovarian cancer. In this study, a detailed structure analysis of the N-linked glycans from total glycoproteins from the SKOV3 ovarian carcinoma cell line and from a recombinantly expressed secretory glycoprotein, erythropoietin (EPO), produced from the same cells has been performed using high-performance anion exchange chromatography with pulsed amperometric detection and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Total cellular N-glycans contained high-mannose type and proximally fucosylated complex type partially agalactosylated structures. On the other hand, the recombinant human EPO secreted from SKOV3 cells contained predominantly core-fucosylated tetraantennary structures, which were partially lacking one or two galactose residues, and partially contained the LacdiNAc motif. Only minor amounts of di- and triantennary complex-type glycans were found, and high-mannose-type glycans were not present in the secreted EPO protein. A large amount of N-acetylneuraminic acid in α2,3-linkage was detected as well. Endogenous glycoproteins were also found to contain the LacdiNAc motif in N-linked glycans. This work contributes to the knowledge of the glycosylation of a human ovarian cancer cell line. It also establishes the basis to further explore high-mannose-type glycans, and the LacdiNAc motif as possible markers of ovarian carcinoma.

• Authors: Eda Machado, Sebastian Kandzia, Rita Carilho, Peter Altevogt, Harald S Conradt, Júlia Costa
• Journal: Glycobiology (Glycobiology) Vol. 21 Issue 3 Pg. 376-86 (Mar 2011) ISSN: 1460-2423 [Electronic] England
• PMID: 21030537 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• Glycoproteins
• Polysaccharides
• Recombinant Proteins
• Erythropoietin
• N-acetylgalactosaminyl-1-4-N-acetylglucosamine
• Lactose
• Mannose

Topics

• Biomarkers, Tumor (metabolism)
• Erythropoietin (biosynthesis, metabolism)
• Female
• Glycoproteins (metabolism)
• Glycosylation
• Humans
• Lactose (analogs & derivatives, metabolism)
• Mannose (metabolism)
• Molecular Structure
• Ovarian Neoplasms
• Polysaccharides (metabolism)
• Recombinant Proteins (biosynthesis, metabolism)
• Tumor Cells, Cultured