Pharmacokinetics of 5-aminofluorescein-albumin, a novel fluorescence marker of brain tumors during surgery.

Abstract

In a phase I/II study, the new fluorescence marker 5-aminofluorescein covalently bound to human serum albumin (AFL-HSA) was intravenously administered to 10 patients with brain tumor 1 to 4 days before surgery, and AFL-HSA kinetics were determined to assess the optimum timing of dye administration. AFL-HSA was determined in serum and brain tissue by size exclusion chromatography with fluorescence detection. AFL-HSA disposition was quite similar to albumin kinetics with small volumes of distribution (volume of central compartment [V(c)] = 2.4 L; volume of distribution [Vd(β)] = 6.8 L; volume at steady state [V(ss)] = 5.9 L), a low clearance (mean Cl = 16.85 mL/h), and a long elimination half-life (mean T(1/2;β) = 307 hours). Predicted peak concentrations in the peripheral compartment occurred at 80.9 hours, the time point of optimal fluorescence intensity observed in brain tumors during surgery.

• Authors: Reinhard Ding, Eva Frei, Mahmoudreza Fardanesh, Hans-Hermann Schrenk, Paul Kremer, Walter E Haefeli
• Journal: Journal of clinical pharmacology (J Clin Pharmacol) Vol. 51 Issue 5 Pg. 672-8 (May 2011) ISSN: 1552-4604 [Electronic] England
• PMID: 20978277 (Publication Type: Clinical Trial, Phase I, Clinical Trial, Phase II, Journal Article)

Chemical References

• Fluoresceins
• Fluorescent Dyes
• Serum Albumin
• 5-aminofluorescein

Topics

• Aged
• Brain Neoplasms (metabolism, surgery)
• Chromatography, Gel
• Chromatography, High Pressure Liquid
• Female
• Fluoresceins (administration & dosage, pharmacokinetics)
• Fluorescent Dyes (administration & dosage, pharmacokinetics)
• Germany
• Half-Life
• Humans
• Infusions, Intravenous
• Male
• Metabolic Clearance Rate
• Middle Aged
• Models, Biological
• Molecular Probe Techniques
• Monitoring, Intraoperative (methods)
• Neurosurgical Procedures
• Serum Albumin (administration & dosage, pharmacokinetics)
• Spectrometry, Fluorescence
• Tissue Distribution