Abstract |
Non-healing bacterial infections are often associated with the formation of a biofilm, where bacteria are more resistant to conventional treatment modalities and to host immune responses. We show here that RNAIII inhibiting peptide (RIP), a linear heptapeptide, is very effective in treating severe polymicrobial infections, including drug-resistant staphylococci like MRSA. By functional genomics studies (microarray analysis) on Staphylococcus aureus, we show here that RIP downregulates the expression of genes involved in biofilm formation and toxin production, and upregulates genes involved in stress response. This pattern of gene regulation may explain why RIP has been so effective in treating severe infections and hopefully through the addition of RIP to existing protocols, a new way of tackling chronic persistent infections will be established.
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Authors | Florencia Lopez-Leban, Madanahally Divakar Kiran, Randall Wolcott, Naomi Balaban |
Journal | The International journal of artificial organs
(Int J Artif Organs)
Vol. 33
Issue 9
Pg. 582-9
(Sep 2010)
ISSN: 1724-6040 [Electronic] United States |
PMID | 20963725
(Publication Type: Case Reports, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Agr protein, Staphylococcus aureus
- Anti-Infective Agents
- Bacterial Proteins
- Oligopeptides
- RNAIII inhibiting peptide
- Trans-Activators
- Virulence Factors
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Topics |
- Anti-Infective Agents
(therapeutic use)
- Bacterial Adhesion
(drug effects)
- Bacterial Proteins
(genetics)
- Biofilms
(drug effects)
- Chronic Disease
- Diabetic Foot
(drug therapy, microbiology, pathology)
- Drug Therapy, Combination
- Gene Expression Profiling
(methods)
- Gene Expression Regulation, Bacterial
(drug effects)
- Humans
- Male
- Methicillin-Resistant Staphylococcus aureus
(drug effects, growth & development)
- Middle Aged
- Oligonucleotide Array Sequence Analysis
- Oligopeptides
(therapeutic use)
- Staphylococcal Infections
(drug therapy, microbiology, pathology)
- Staphylococcus aureus
(drug effects, genetics, growth & development, pathogenicity)
- Stress, Physiological
(genetics)
- Surgical Wound Infection
(drug therapy, microbiology, pathology)
- Time Factors
- Trans-Activators
(genetics)
- Treatment Outcome
- Virulence Factors
(genetics)
- Wound Healing
(drug effects)
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