[Effects of the spider venom on proliferation of human lung adenocarcinoma cell A549].

Abstract

BACKGROUND AND OBJECTIVE:

The spider venom may inspire new drugs to treat cancer. The aim of this study is to investigate the effects and mechanisms of spider venom on lung adenocarcinoma cell A549.

METHODS:

The proliferation of lung adenocarcinoma A549 cells was detected by MTT. The apoptosis rate was observed with MTT assay and flow cytometer. The activity of catalase was detected by colorimetry. The malondialdehyde (MDA) content was determined by improved thiobarbituric acid fluorometric method. The expression of P38MAPK protein was analyzed with Western blot.

RESULTS:

Spider venom can remarkably inhibite the proliferation of lung adenocarcinoma A549 cells, increased activity of catalase and MDA content, down-regulated expression of P38MAPK compared with the control group.

CONCLUSIONS:

The reduced proliferation of lung adenocarcinoma A549 cells by spider venom is may be associated with the increased of activity of catalase and MDA content and decreased expression of P38MAPK.

• Authors: Zengxiang Hu, Yulei Du, Quanxi Liu, Yuan Wang, Liang Li
• Journal: Zhongguo fei ai za zhi = Chinese journal of lung cancer (Zhongguo Fei Ai Za Zhi) Vol. 13 Issue 10 Pg. 933-6 (Oct 2010) ISSN: 1999-6187 [Electronic] China
• PMID: 20959064 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Spider Venoms
• Malondialdehyde
• Catalase
• p38 Mitogen-Activated Protein Kinases

Topics

• Adenocarcinoma (enzymology, genetics, metabolism, pathology)
• Adenocarcinoma of Lung
• Antineoplastic Agents (pharmacology)
• Apoptosis (drug effects)
• Catalase (metabolism)
• Cell Division (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• G2 Phase (drug effects)
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Lung Neoplasms (enzymology, genetics, metabolism, pathology)
• Malondialdehyde (metabolism)
• Spider Venoms (pharmacology)
• p38 Mitogen-Activated Protein Kinases (metabolism)