[Effect of oral glutamine on intestinal barrier function in young rats with endotoxemia].

Abstract

OBJECTIVE:

To study the effect of glutamine on intestinal barrier function by examining the changes of plasma D-lactic levels and diamine oxidase (DAO) levels in plasma and intestinal tissue after glutamine intervention in young rats with endotoxemia.

METHODS:

Eighty 18-day-old rats were randomly divided into endotoxemia and glutamine intervention groups (n=40 each). Endotoxemia was induced by lipopolysaccharide (LPS) injection. Plasma and small intestine homogenate were collected 1.5, 6, 24 and 72 hrs and 7 days after LPS injection. The glutamine intervention group was immediately administered with oral glutamine (2 g/kg) after LPS injection. Afterwards, glutamine was administered once daily. Plasma D-lactic and DAO levels and intestinal DAO levels were measured.

RESULTS:

Plasma DAO activity in the glutamine intervention group was significantly lower than that in the endotoxemia group 6 and 72 hrs after LPS injection (P<0.05). In contrast, the intestinal DAO activity in the glutamine intervention group was significantly higher than that in the endotoxemia group 6, 24 and 72 hrs and 7 days after LPS injection (P<0.05 or 0.01). Plasma D-lactic levels in the glutamine intervention group were significantly lower than those in the endotoxemia group 6, 24 and 72 hrs and 7 days after LPS injection (P<0.01).

CONCLUSIONS:

Glutamine may reduce the permeability of intestinal mucosa, and thus provides protective effects on intestinal barrier function in rats with endotoxemia.

• Authors: Xiao Chang, Lin-Lin Wang, Shu-Jun Lian, Qing Tang, Ping Chen, Hua Wang
• Journal: Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics (Zhongguo Dang Dai Er Ke Za Zhi) Vol. 12 Issue 10 Pg. 809-11 (Oct 2010) ISSN: 1008-8830 [Print] China
• PMID: 20959048 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Glutamine
• Lactic Acid
• Amine Oxidase (Copper-Containing)

Topics

• Administration, Oral
• Amine Oxidase (Copper-Containing) (metabolism)
• Animals
• Behavior, Animal
• Endotoxemia (metabolism)
• Female
• Glutamine (administration & dosage, pharmacology)
• Intestinal Mucosa (metabolism)
• Intestines (drug effects)
• Lactic Acid (blood)
• Male
• Rats
• Rats, Wistar