Abstract |
Quantum dots (QDs) have shown great development potential in noninvasive imaging and monitoring of cancer cells in vivo because of their unique optical properties. However, the key issue of whether or not QDs-labeled cancer cells affect the proliferation, apoptosis and in vivo tumorigenicity ability has not been reported. The primary issue is if the results obtained from the noninvasive visualization of QDs-labeled tumors are scientific. Here, we applied peptide-linked near-conjugated fluorescent QDs to label human buccal squamous cell carcinoma cell line (BcaCD885). We performed in vivo tumorigenicity ability assays, tumorigenic cells proliferation, and apoptotic capability assays detected by flow cytometry and plate clone formation experiment, and found that peptide-linked near-conjugated fluorescent QDs labeling did not affect the growth, proliferation, apoptosis, and tumorigenicity ability of those cancer cells. Our study provides scientific foundation to support the application of near-infrared fluorescent QDs in noninvasive imaging and monitoring of cancer cells in vivo.
|
Authors | Deping Sun, Kai Yang, Gang Zheng, Zhigang Li, Yuan Cao |
Journal | International journal of nanomedicine
(Int J Nanomedicine)
Vol. 5
Pg. 401-5
(Aug 09 2010)
ISSN: 1178-2013 [Electronic] New Zealand |
PMID | 20957161
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
|
Topics |
- Animals
- Apoptosis
- Carcinoma, Squamous Cell
(pathology)
- Cell Line, Tumor
- Cell Proliferation
- Cheek
- Female
- Fluorescence
- Humans
- Infrared Rays
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- Mouth Neoplasms
(pathology)
- Nanomedicine
- Neoplasm Transplantation
- Peptides
- Quantum Dots
- Transplantation, Heterologous
- Tumor Stem Cell Assay
|