Identification of ester glucuronide and sulfate conjugates of 5-hydroxy-6-methoxyindole-2-carboxylic acid and 6-hydroxy-5-methoxyindole-2-carboxylic acid in melanoma urine.

Abstract

HPLC analysis of urine sample from a patient with wide-spread melanoma revealed the presence of unknown indolic compounds at extraordinarily high levels, detectable with electrochemical and/or fluorescent detectors. By enzymic and chemical hydrolyses, they were identified as ester glucuronide and sulfate conjugates of 5-hydroxy-6-methoxyindole-2-carboxylic acid and 6-hydroxy-5-methoxyindole-2-carboxylic acid. Urine samples from B16 melanoma-bearing mice contained the sulfate conjugates but not the ester glucuronide conjugates.

• Authors: K Wakamatsu, S Ito
• Journal: Journal of dermatological science (J Dermatol Sci) Vol. 1 Issue 4 Pg. 253-9 (Jul 1990) ISSN: 0923-1811 [Print] Netherlands
• PMID: 2095194 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Glucuronates
• Indoles
• Sulfates
• 5-hydroxy-6-methoxy-2-indolylcarboxylic acid
• 6-hydroxy-5-methoxy-2-indolylcarboxylic acid

Topics

• Animals
• Chromatography, High Pressure Liquid
• Glucuronates (urine)
• Humans
• Indoles (urine)
• Melanoma (urine)
• Mice
• Skin Neoplasms (urine)
• Sulfates (metabolism)