Abstract | BACKGROUND: METHOD: Plasma samples from 7 healthy volunteers and 27 HBV infected patients with different stages of fibrosis were selected for 2-DIGE proteomic screening. One-way ANOVA analysis was used to assess differences in protein expression among all groups. The alteration was further confirmed by western blotting. Plasma levels of 25 serological variables in 42 healthy volunteers and 68 patients were measured to establish a decision tree for the detection of various stages fibrosis. RESULT: The up-regulated proteins along with fibrosis progress included fibrinogen, collagen, macroglobulin, hemopexin, antitrypsin, prealbumin and thioredoxin peroxidase. The down-regulated proteins included haptoglobin, serotransferrin, CD5 antigen like protein, clusterin, apolipoprotein and leucine-rich alpha-2-glycoprotein. For the discrimination of milder stage fibrosis, the area under curve for Prx II was the highest. Four variables (PT, Pre, HA and Prx II) were selected from the 25 variables to construct the decision tree. In a training group, the correct prediction percentage for normal control, milder fibrosis, significant fibrosis and early cirrhosis was 100%, 88.9%, 95.2% and 100%, respectively, with an overall correct percent of 95.9%. CONCLUSION: This study showed that 2-D DIGE-based proteomic analysis of the plasma was helpful in screening for new plasma biomarkers for liver disease. The significant up-expression of Prx II could be used in the early diagnosis of HBV related liver fibrosis.
|
Authors | Ye Lu, Jie Liu, Chengzhao Lin, Haijian Wang, Ying Jiang, Jiyao Wang, Pengyuan Yang, Fuchu He |
Journal | BMC gastroenterology
(BMC Gastroenterol)
Vol. 10
Pg. 115
(Oct 13 2010)
ISSN: 1471-230X [Electronic] England |
PMID | 20939925
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Biomarkers
- Peroxiredoxins
|
Topics |
- Adult
- Biomarkers
(blood)
- Blotting, Western
- Early Diagnosis
- Electrophoresis, Gel, Two-Dimensional
- Enzyme-Linked Immunosorbent Assay
- Female
- Hepatitis B, Chronic
(complications, diagnosis, enzymology)
- Humans
- Liver Cirrhosis
(diagnosis, enzymology, virology)
- Male
- Peroxiredoxins
(blood)
- Prognosis
- Proteomics
(methods)
- Retrospective Studies
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
|