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Cloning, expression, and characterization of Der f 7, an allergen of Dermatophagoides farinae from China.

Abstract
A full-length cDNA encoding house dust mite allergen Der f 7 from Dermatophagoides farina (Acari: Pyroglyphidae) from China was cloned, sequenced, and successfully expressed. A reference sequence (GenBank accession AY283292) was used to design polymerase chain reaction primers. Analysis revealed eight mismatched nucleotides in five Der f 7 cDNA clones, and the projected amino acid sequence contained six incompatible residues. These results suggest that the sequence of Der f 7 may be polymorphic. Further bioinformatic analysis revealed that the mature Der f 7 allergen had a molecular mass of approximately 21.88 kDa and a theoretical isoelectric point of 4.90. Der f 7 protein secondary structure was composed of a helix (56.63%), extended strand (5.10%), and random coil (38.27%). Group 7 allergens are present in Pyroglyphidae, Acaridae, and Glycyphagidae families, and homology analysis revealed a 86% similarity between Der f 7 and Der p 7. Furthermore, a phylogenetic tree constructed of group 7 allergens from different mite species revealed that Der f 7 and Der p 7 clustered with 100% bootstrap support. Bioinformatics-driven characterization of Der f 7 allergen as conducted in this study may contribute to diagnostic and therapeutic applications for dust mite allergies.
AuthorsYu-bao Cui, Hong-xing Cai, Ying Zhou, Cui-xiang Gao, Wei-hong Shi, Ming Yu, Li Li
JournalJournal of medical entomology (J Med Entomol) Vol. 47 Issue 5 Pg. 868-76 (Sep 2010) ISSN: 0022-2585 [Print] United States
PMID20939383 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antigens, Dermatophagoides
  • Arthropod Proteins
  • Dermatophagoides farinae antigen f 7
Topics
  • Amino Acid Sequence
  • Animals
  • Antigens, Dermatophagoides (genetics, metabolism)
  • Arthropod Proteins
  • Base Sequence
  • China
  • Cloning, Molecular
  • Dermatophagoides farinae (immunology, metabolism)
  • Gene Expression Regulation (genetics, physiology)
  • Molecular Sequence Data
  • Phylogeny

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