Abstract |
A full-length cDNA encoding house dust mite allergen Der f 7 from Dermatophagoides farina (Acari: Pyroglyphidae) from China was cloned, sequenced, and successfully expressed. A reference sequence (GenBank accession AY283292) was used to design polymerase chain reaction primers. Analysis revealed eight mismatched nucleotides in five Der f 7 cDNA clones, and the projected amino acid sequence contained six incompatible residues. These results suggest that the sequence of Der f 7 may be polymorphic. Further bioinformatic analysis revealed that the mature Der f 7 allergen had a molecular mass of approximately 21.88 kDa and a theoretical isoelectric point of 4.90. Der f 7 protein secondary structure was composed of a helix (56.63%), extended strand (5.10%), and random coil (38.27%). Group 7 allergens are present in Pyroglyphidae, Acaridae, and Glycyphagidae families, and homology analysis revealed a 86% similarity between Der f 7 and Der p 7. Furthermore, a phylogenetic tree constructed of group 7 allergens from different mite species revealed that Der f 7 and Der p 7 clustered with 100% bootstrap support. Bioinformatics-driven characterization of Der f 7 allergen as conducted in this study may contribute to diagnostic and therapeutic applications for dust mite allergies.
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Authors | Yu-bao Cui, Hong-xing Cai, Ying Zhou, Cui-xiang Gao, Wei-hong Shi, Ming Yu, Li Li |
Journal | Journal of medical entomology
(J Med Entomol)
Vol. 47
Issue 5
Pg. 868-76
(Sep 2010)
ISSN: 0022-2585 [Print] England |
PMID | 20939383
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Dermatophagoides
- Arthropod Proteins
- Dermatophagoides farinae antigen f 7
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Topics |
- Amino Acid Sequence
- Animals
- Antigens, Dermatophagoides
(genetics, metabolism)
- Arthropod Proteins
- Base Sequence
- China
- Cloning, Molecular
- Dermatophagoides farinae
(immunology, metabolism)
- Gene Expression Regulation
(genetics, physiology)
- Molecular Sequence Data
- Phylogeny
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