Biomarker expression in lung of rabbit with pulmonary fibrosis induced by ammonium perchlorate.

Abstract

Ammonium perchlorate (AP), an oxidizer, has been used in solid propellants. Although AP exposure has been suspected as a risk factor for the development of pulmonary fibrosis, data are still inconclusive. To evaluate the pulmonary toxicity and the potential pulmonary fibrosis caused by occupational exposure to this compound, 25 male rabbits were randomly allocated into five groups to receive AP or bleomycin or saline by intratracheal injection. All rabbits were sacrificed and total RNA from the lungs was extracted. Expressions of types I and III collagens, transforming growth factor-β(1) (TGF-β(1)) and tumour necrosis factor-α (TNF-α) messenger RNA (mRNA) were measured by reverse transcription-polymerase chain reaction (RT-PCR). The expressions of type I and III collagen mRNA in low, moderate and high dose AP groups were significantly higher (p < 0.01 or p < 0.05) than that in the saline group. There was also a significant increased level of TGF-β(1) and TNF-α mRNA in the three AP groups compared with saline control group (p < 0.01 or p < 0.05). These results reveal that AP can increase gene expressions of types I, III collagens, TGF-β(1) and TNF-α in lung of rabbits exposed to AP. The overexpression of these biomarkers were considered as effective indicator linking to the development of pulmonary fibrosis and finally demonstrated that AP has potential to induce pulmonary fibrosis.

• Authors: Feng-hong Wu, Hui-xia Guo, Ming-fang Lin, Zhi-ze Chen, Xuan Zhou, Kai-liang Peng
• Journal: Toxicology and industrial health (Toxicol Ind Health) Vol. 27 Issue 3 Pg. 235-41 (Apr 2011) ISSN: 1477-0393 [Electronic] England
• PMID: 20937628 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Air Pollutants, Occupational
• Biomarkers
• Collagen Type I
• Collagen Type III
• Genetic Markers
• Perchlorates
• Quaternary Ammonium Compounds
• RNA, Messenger
• Transforming Growth Factor beta1
• Tumor Necrosis Factor-alpha
• Bleomycin
• ammonium perchlorate

Topics

• Air Pollutants, Occupational (toxicity)
• Animals
• Biomarkers (metabolism)
• Bleomycin (toxicity)
• Collagen Type I (genetics, metabolism)
• Collagen Type III (genetics, metabolism)
• Disease Models, Animal
• Gene Expression (drug effects)
• Genetic Markers (drug effects, genetics)
• Intubation, Intratracheal
• Male
• Perchlorates (toxicity)
• Pulmonary Fibrosis (chemically induced, genetics, metabolism)
• Quaternary Ammonium Compounds (toxicity)
• RNA, Messenger (metabolism)
• Rabbits
• Transforming Growth Factor beta1 (genetics, metabolism)
• Tumor Necrosis Factor-alpha (genetics, metabolism)