Abstract | BACKGROUND AND AIMS: Lysosomes play an important role in acute pancreatitis (AP). Here we developed a method for the isolation of lysosome subpopulations from rat pancreas and assessed the stability of lysosomal membranes. METHODS: RESULTS: AP was associated with: (i) increases in the specific activity of lysosomal enzymes in the soluble fraction, (ii) changes in the size and alterations in the morphology of the organelles from the lysosomal subpopulations, (iii) the appearance of large vacuoles in the primary and secondary lysosome subpopulations, (iv) the increase in the amount of the NAG form associated with the pancreatic lysosomal membrane as well as its release towards the soluble fraction. CONCLUSIONS: Lysosome subpopulations are separated by a combination of differential and Percoll density gradient centrifugations. Primary lysosome membrane stability decreases in AP.
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Authors | Nancy Sarmiento, Jesús Sánchez-Yagüe, Pedro P Juanes, Nieves Pérez, Laura Ferreira, Violeta García-Hernández, Arturo Mangas, José J Calvo, Carmen Sánchez-Bernal |
Journal | Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver
(Dig Liver Dis)
Vol. 43
Issue 2
Pg. 132-8
(Feb 2011)
ISSN: 1878-3562 [Electronic] Netherlands |
PMID | 20934396
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2010 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved. |
Chemical References |
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Topics |
- Acute Disease
- Animals
- Ceruletide
- Disease Models, Animal
- Lysosomes
(pathology)
- Male
- Pancreatitis
(chemically induced, enzymology, pathology)
- Rats
- Rats, Wistar
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