In vitro and in vivo antioxidant properties of chlorogenic acid and caffeic acid.

Abstract

Dietary polyphenols are thought to be beneficial for human health as antioxidants. Coffee beans contain a common polyphenol, chlorogenic acid. Chlorogenic acid is the ester of caffeic acid and quinic acid. Although these polyphenols have received much attention, there is little evidence indicating a relationship between the effect and the rate of absorption. In this study, we focused on the beneficial effects of chlorogenic acid and caffeic acid, a major metabolite of chlorogenic acid. We carried out in vitro and in vivo experiments. In the in vitro study, caffeic acid had stronger antioxidant activity than that of chlorogenic acid. The uptake of chlorogenic acid by Caco-2 cells was much less than that of caffeic acid. The physiological importance of an orally administered compound depends on its availability for intestinal absorption and subsequent interaction with target tissues. We then used an intestinal ischemia-reperfusion model to evaluate antioxidant activities in vivo. We found that both chlorogenic acid and caffeic acid had effects on intestinal ischemia-reperfusion injury. Since caffeic acid has a stronger antioxidant activity than that of chlorogenic acid and chlorogenic acid is hydrolyzed into caffeic acid in the intestine, it is possible that caffeic acid plays a major role in the protective effect of chlorogenic acid against ischemia-reperfusion injury.

• Authors: Yuki Sato, Shirou Itagaki, Toshimitsu Kurokawa, Jiro Ogura, Masaki Kobayashi, Takeshi Hirano, Mitsuru Sugawara, Ken Iseki
• Journal: International journal of pharmaceutics (Int J Pharm) Vol. 403 Issue 1-2 Pg. 136-8 (Jan 17 2011) ISSN: 1873-3476 [Electronic] Netherlands
• PMID: 20933071 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2010 Elsevier B.V. All rights reserved.

Chemical References

• Antioxidants
• Caffeic Acids
• Superoxides
• Chlorogenic Acid
• caffeic acid

Topics

• Animals
• Antioxidants (chemistry, pharmacokinetics, pharmacology, therapeutic use)
• Caco-2 Cells
• Caffeic Acids (chemistry, pharmacokinetics, pharmacology, therapeutic use)
• Capillary Permeability
• Chlorogenic Acid (chemistry, pharmacokinetics, pharmacology, therapeutic use)
• Humans
• Intestinal Absorption
• Intestine, Small (blood supply, drug effects, metabolism)
• Male
• Oxidation-Reduction
• Rats
• Rats, Wistar
• Reperfusion Injury (metabolism, prevention & control)
• Superoxides (analysis)