Abstract | AIM OF THE STUDY: METHODS: Seventy-two adult male rats were randomized to receive haemorrhagic shock/ resuscitation (HS), HS plus platonin (10, 50, or 100μg/kg intravenous injection immediately after resuscitation), sham instrumentation ( Sham), or Sham plus platonin (100μg/kg) (n=12 in each group). Haemorrhagic shock was induced by blood drawing and mean blood pressure was maintained at 40-45mmHg for 120min. Then, resuscitation was achieved by shed blood/saline mixtures re-infusion. After monitoring for another 8h, rats were sacrificed. RESULTS: Arterial blood gas and histological findings, in concert with assays of leukocyte infiltration (polymorphonuclear leukocytes/alveoli ratio and myeloperoxidase activity) and lung water content (wet/dry weight ratio), confirmed that haemorrhagic shock/ resuscitation caused significant lung injury. Significant increases in concentrations of inflammatory molecules ( chemokine, cytokine, and prostaglandin E(2)) as well as nitric oxide and malondialdehyde in lung tissues confirmed that haemorrhagic shock/ resuscitation elicited inflammatory response and imposed oxidative stress in rats. Platonin at the dosages of 50 and 100μg/kg, but not 10μg/kg, significantly attenuated the inflammatory response and oxidative stress induced by haemorrhagic shock/ resuscitation. Most important, platonin at the dosages of 50 and 100μg/kg, but not 10μg/kg, significantly mitigated the lung injury induced by haemorrhagic shock/ resuscitation. CONCLUSIONS:
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Authors | Hsi-Ning Chu, Pei-Shan Tsai, Tao-Yeuan Wang, Chun-Jen Huang |
Journal | Resuscitation
(Resuscitation)
Vol. 82
Issue 1
Pg. 97-104
(Jan 2011)
ISSN: 1873-1570 [Electronic] Ireland |
PMID | 20932630
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2010 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
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Topics |
- Acute Lung Injury
(etiology, prevention & control)
- Animals
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Injections, Intravenous
- Male
- Rats
- Rats, Sprague-Dawley
- Resuscitation
(methods)
- Shock, Hemorrhagic
(complications, therapy)
- Thiazoles
(administration & dosage, therapeutic use)
- Treatment Outcome
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