[Dracorhodin perchlorate inhibit high glucose induce serum and glucocorticoid induced protein kinase 1 and fibronectin expression in human mesangial cells].

Abstract

OBJECTIVE:

To investigate the effect of dracorhodin perchlorate (DP) on inhibiting high glucose-induced serum and glucocorticoid induced protein kinase 1 (SGK1) and fibronectin (FN) expression in human mesangial cells (HMC), and its mechanism of prevention and treatment on renal fibrosis in diabetic nephropathy (DN) .

METHOD:

The HMC were divided into normal glucose group (NG group, 5.5 mmol x L(-1) D-glucose), normal glucose +low DP group (NG + LDP group, 5.5 mmol x L(-1) D-glucose +7.5 micromol x L(-1) DP), normal glucose +high DP group (NG + HDP group, 5.5 mmol x L(-1) D-glucose + 15 micromol x L(-1) DP), high glucose group (HG group,25 mmol x L(-1) D-glucose), high glucose +low DP group (HG + LDP group, 25 mmol x L(-1) D-glucose + 7.5 micromol x L(-1) DP)and high glucose +high DP group (HG +HDP group, 25 mmol x L(-1) D-glucose + 15 micromol x L(-1) DP). Each group was examined at 24 hours. The levels of SGK1 and FN mRNA was detected by real-time fluorescence quantitative PCR,and the expression of SGK1 and FN protein was detected by Western blot or indirect immunofluorescence.

RESULT:

A basal level of SGK1 and FN in HMC were detected in NG group, and the level of SGK1 and FN mRNA and protein were not evidently different compared to that of NG group adding 7.5 micromol x L(-1) DP for 24 hours. On the other hand, the levels of SGK1 and FN mRNA and protein were obviously decreased by adding 15 micromol x L(-1) DP for 24 hours. Compared to NG group, the levels of SGK1 and FN mRNA and protein were increased in HG group after stimulating for 24 hours (P < 0.01). Compared to HG group, the level of SGK1 and FN mRNA and protein were evidently reduced in HG + LDP and HG + HDP groups by adding 7.5 micromol x L(-1) DP and 15 micromol x L(-1) DP for 24 hours (P < 0.01).

CONCLUSION:

Dracorhodin perchlorate can inhibit high glucose-induced serum and glucocorticoid induced protein kinase 1 (SGK1) and fibronectin(FN) expression in human mesangial cells, and this may be part of the mechanism of preventing and treating renal fibrosis of DN.

• Authors: Yifeng Xie, Quansheng Wang, Jianguo Liu, Jiwen Xie, Kaming Xue, Qing Tang
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 35 Issue 15 Pg. 1996-2000 (Aug 2010) ISSN: 1001-5302 [Print] China
• PMID: 20931854 (Publication Type: Journal Article)

Chemical References

• Benzopyrans
• Drugs, Chinese Herbal
• Fibronectins
• Immediate-Early Proteins
• Perchlorates
• dracorhodin
• Protein Serine-Threonine Kinases
• serum-glucocorticoid regulated kinase
• Glucose
• perchlorate

Topics

• Benzopyrans (pharmacology)
• Cell Line
• Diabetic Nephropathies (drug therapy, enzymology, genetics, metabolism)
• Down-Regulation (drug effects)
• Drugs, Chinese Herbal (pharmacology)
• Fibronectins (biosynthesis, genetics)
• Gene Expression (drug effects)
• Glucose (metabolism)
• Humans
• Immediate-Early Proteins (genetics, metabolism)
• Mesangial Cells (drug effects, enzymology, metabolism)
• Perchlorates (pharmacology)
• Protein Serine-Threonine Kinases (genetics, metabolism)