Abstract | BACKGROUND: In an evaluation of androstenedione results from patient serum samples using the Siemens Immulite 2500 analyser and manual Coat-A-Count (CAC) methods, three outliers were evident with grossly elevated results in the CAC assay. METHODS: RESULTS:
Spironolactone treatment was associated with higher androstenedione concentrations measured by the CAC assay that reverted to normal on treatment withdrawal. Based on a single test with spironolactone at 1000 ng/mL, the manufacturer reported only 0.109% interference in the CAC assay. CONCLUSIONS:
Spironolactone (and/or its metabolites) may interfere in the Siemens CAC assay for androstenedione but not in the Immulite 2500 assay. This experience highlights the need for information from clinicians on drug treatment when laboratory investigations are requested. Drug interferences in immunoassay are common and need evaluation beyond tests performed to certify laboratory reagents.
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Authors | J W Honour, T Tsilchorozidou, G S Conway, A Dawnay |
Journal | Annals of clinical biochemistry
(Ann Clin Biochem)
Vol. 47
Issue Pt 6
Pg. 564-6
(Nov 2010)
ISSN: 1758-1001 [Electronic] England |
PMID | 20926464
(Publication Type: Journal Article)
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Chemical References |
- Spironolactone
- Androstenedione
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Topics |
- Androstenedione
(blood)
- Female
- Humans
- Immunoassay
(methods)
- Polycystic Ovary Syndrome
(blood, drug therapy)
- Spironolactone
(blood, metabolism, therapeutic use)
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