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Gd-EOB-DTPA enhanced micro-MR imaging of hepatic tumors in H-ras 12V transgenic mice.

AbstractRATIONALE AND OBJECTIVES:
The aims of this study were to evaluate the morphologic characteristics and growth pattern of hepatic tumors in H-ras 12V transgenic (TG) mice using a micro-magnetic resonance (MR) system and to assess the usefulness of gadolinium ethoxybenzyl diethylenetriamine penta-acetic acid (Gd-EOB-DTPA) enhancement for the detection of hepatic tumors in these mice.
MATERIALS AND METHODS:
Hepatocellular carcinoma lines were established to allow insertion of the H-ras 12V transgene under the control of the albumin enhancer/promoter. Seven H-ras 12V TG mice and four wild-type mice were included in this study. The mice underwent various MR imaging examinations, including T1-weighted imaging (repetition time, 300 ms; echo time, 11 ms), Gd-EOB-DTPA-enhanced T1-weighted imaging (dose, 0.025 mmol/kg), and T2-weighted imaging (repetition time, 3500 ms; echo time, 36 ms), with a 4.7-T MR scanner, at 4, 6, 8, and 9 months of age. All mice were euthanized after the final MR imaging procedure, except for one TG mouse and two wild-type mice that were euthanized after MR imaging procedures at 4 months of age. For imaging analysis, the tumor characteristics in each MR sequence, including tumor size, number, and signal intensity (SI), were recorded, and the contrast-to-noise ratio and contrast enhancement ratio were calculated to quantify the SI of the tumor. The MR images were correlated with the findings of histopathologic examinations.
RESULTS:
No tumors were detected in the four wild-type mice. In the six TG mice, a total of 67 tumors were found in histopathologic specimens obtained at 9 months of age. Of the 67 tumors, 62 were detected on Gd-EOB-DTPA-enhanced T1-weighted images with fat saturation. The majority of hepatic tumors showed high SI on T1-weighted images without fat saturation. The SI diminished on T1-weighted images with fat saturation. The tumor contrast-to-noise ratio for Gd-EOB-DTPA-enhanced T1-weighted imaging was significantly better than that for the other sequences. The tumors were histopathologically confirmed as hepatocellular adenomas (n = 32) and well-differentiated hepatocellular carcinomas (n = 35).
CONCLUSIONS:
Micro-MR imaging can reveal the characteristics of hepatic tumors in a live murine model. Gd-EOB-DTPA-enhanced T1-weighted imaging is helpful in the detection of hepatic tumors in H-ras 12V TG mice.
AuthorsDong-Ho Bang, Seong-Hoon Park, Hong Young Jun, Hyung-Bae Moon, Sun-Uk Kim, Dae-Yeul Yu, Kwon-Ha Yoon
JournalAcademic radiology (Acad Radiol) Vol. 18 Issue 1 Pg. 13-9 (Jan 2011) ISSN: 1878-4046 [Electronic] United States
PMID20926317 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2011 AUR. All rights reserved.
Chemical References
  • Contrast Media
  • gadolinium ethoxybenzyl DTPA
  • Gadolinium DTPA
Topics
  • Animals
  • Carcinoma, Hepatocellular (pathology)
  • Contrast Media
  • Diagnosis, Differential
  • Disease Models, Animal
  • Gadolinium DTPA
  • Genes, ras
  • Image Enhancement (methods)
  • Liver (pathology)
  • Liver Neoplasms, Experimental (pathology)
  • Magnetic Resonance Imaging (methods)
  • Mice
  • Mice, Transgenic
  • Observer Variation

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