Salidroside promotes erythropoiesis and protects erythroblasts against oxidative stress by up-regulating glutathione peroxidase and thioredoxin.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Rhodiola rosea is commonly used in China and Tibet folk medicine for the treatment of high altitude sickness, anoxia and mountain malhypoxia.

AIM OF STUDY:

Salidroside (SDS) is an active ingredient of Rhodiola rosea. This study attempted to examine the potential erythropoiesis-stimulating and anti-oxidative effect of SDS in TF-1 erythroblasts.

MATERIALS AND METHODS:

The erythropoiesis-promoting effect was determined by treating human TF-1 cells, one of the popular in vitro models for studying erythropoiesis, with SDS in the presence and absence of erythropoietin (EPO) through the measurement of the expression of a series of erythroid markers such as glycophorin A (GPA), transferrin receptor (CD71) and hemoglobin (Hb). The potential protective effect of SDS against H(2)O(2)-induced apoptosis and its underlying mechanism in TF-1 erythroblasts were examined by flow cytometry and Western blot analysis.

RESULTS:

SDS promotes erythropoiesis in the EPO-treated cells and it also reduces the number of apoptotic cells in TF-1 erythroblasts after H(2)O(2) treatment probably through the up-regulation of protective proteins thioredoxin-1 (Trx1) and glutathione peroxidase-1 (GPx1).

CONCLUSION:

Our study provides evidence to explain the ethnopharmacological role of SDS and Rhodiola rosea in Chinese medicine. Our findings also support the use of SDS as an erythropoiesis-adjuvant agent to correct anemia and malhypoxia.

• Authors: Erin Wei Qian, Daniel Tianfang Ge, Siu-Kai Kong
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 133 Issue 2 Pg. 308-14 (Jan 27 2011) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 20920561 (Publication Type: Journal Article)
• Copyright: Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Glucosides
• Phenols
• Reactive Oxygen Species
• Thioredoxins
• Glutathione Peroxidase
• rhodioloside
• Glutathione Peroxidase GPX1

Topics

• Altitude Sickness (drug therapy)
• Cell Line
• Cell Proliferation (drug effects)
• Erythroblasts (drug effects, metabolism)
• Erythropoiesis (drug effects)
• Ethnopharmacology
• Glucosides (pharmacology)
• Glutathione Peroxidase (metabolism)
• Humans
• Medicine, Chinese Traditional
• Medicine, Tibetan Traditional
• Oxidative Stress (drug effects)
• Phenols (pharmacology)
• Phytotherapy
• Plants, Medicinal (chemistry)
• Reactive Oxygen Species (metabolism)
• Rhodiola (chemistry)
• Thioredoxins (metabolism)
• Up-Regulation (drug effects)
• Glutathione Peroxidase GPX1