Enzymes that hydrolyze adenine nucleotides in patients with ischemic heart disease.

Abstract

BACKGROUND:

The extracellular nucleotides, ATP and ADP, as well as the nucleoside adenosine have been implicated in a great number of pathologic and physiological functions. However, extracellular adenine nucleotide levels are controlled by a complex cell surface-located group of enzymes called ectonucleotidases. We evaluated activities of enzymes that hydrolyze adenine nucleotides and nucleosides in platelets from patients with ischemic heart disease (IHD).

METHODS:

Sixty IHD patients were selected for the study. The activities of ectonucleoside triphosphate diphosphohydrolase (NTPDase, CD39), ectonucleotide pyrophosphatase/phosphodiesterase (E-NPP), ecto-5'-nucleotidase and adenosine deaminase (ADA) were studied in isolated platelets of these patients, as well as the platelet aggregation and NTPDase expression.

RESULTS:

The results show that NTPDase, ecto-5'-nucleotidase, E-NPP activities and NTPDase expression were increased in platelets of IHD patients when compared with the control group (p < 0.05). On the other hand, ADA activity and platelet aggregation were decreased in IHD patients, when compared with the control group (p < 0.05).

CONCLUSIONS:

The pathological condition in IHD generates alterations in ectonucleotidase activities as a compensatory organic response to thrombotic events that occur in IHD.

• Authors: Margarete D Bagatini, Caroline C Martins, Diogo Gasparetto, Roselia M Spanevello, Lara V Becker, Cintia S Rosa, Vanessa Battisti, Luziane Bellé, Jamile F Gonçalves, Maria Rosa C Schetinger, Romualdo B Dos Santos, Liliane Z Oliveira, Vera Maria Morsch
• Journal: Clinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 412 Issue 1-2 Pg. 159-64 (Jan 14 2011) ISSN: 1873-3492 [Electronic] Netherlands
• PMID: 20920495 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2010 Elsevier B.V. All rights reserved.

Chemical References

• Adenine Nucleotides

Topics

• Adenine Nucleotides (metabolism)
• Blood Platelets (enzymology, metabolism)
• Female
• Humans
• Hydrolysis
• Male
• Middle Aged
• Myocardial Ischemia (enzymology, metabolism, physiopathology)
• Platelet Aggregation