The porcine MUC20 gene: molecular characterization and its association with susceptibility to enterotoxigenic Escherichia coli F4ab/ac.

Abstract

Enterotoxigenic Eschoerchia coli (ETEC) F4 is a major cause of diarrhea in neonatal and young pigs. The locus encoding intestinal receptor for ETEC F4ab/ac has been mapped to pig chromosome (SSC) 13q41. We previously assigned mucin 20 (MUC20) to SSC13q41 and considered it a candidate for the ETEC F4ab/ac receptor. In this study, the full-length mRNA sequence of the porcine MUC20 gene was determined, revealing two MUC20 alternative transcripts differing in an 18-bp deletion in the open reading frame (ORF). The longer isoform is 1,927-bp long and contains a 1,572-bp ORF encoding a protein of 523 amino acids. RT-PCR analysis showed that MUC20 mRNA was expressed most highly in the kidney, prostate, epididymis and bladder. Seven single nucleotide polymorphisms in MUC20 were identified in founder animals of a White Duroc × Erhualian cross, and the entire cross population (n = 842) was genotyped for two MUC20 polymorphisms: g.191C>T in intron 5 and c.1600C>T in exon 6. The transmission disequilibrium test (TDT) results revealed that both polymorphisms were significantly associated with in vitro ETEC F4ab/ac adhesion phenotypes in the intercross population, and the most significant association was found between g.191C>T and F4ac adhesion phenotype (P = 1.02 × 10(-9)).

• Authors: Huayuan Ji, Jun Ren, Xueming Yan, Xiang Huang, Bo Zhang, Zhiyan Zhang, Lusheng Huang
• Journal: Molecular biology reports (Mol Biol Rep) Vol. 38 Issue 3 Pg. 1593-601 (Mar 2011) ISSN: 1573-4978 [Electronic] Netherlands
• PMID: 20890662 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Complementary
• Mucins
• RNA, Messenger

Topics

• Alleles
• Alternative Splicing (genetics)
• Animals
• Crosses, Genetic
• DNA, Complementary (genetics)
• Enterotoxigenic Escherichia coli (physiology)
• Escherichia coli Infections (genetics, veterinary)
• Female
• Gene Expression Profiling
• Genetic Predisposition to Disease
• Haplotypes (genetics)
• Linkage Disequilibrium (genetics)
• Male
• Molecular Sequence Data
• Mucins (genetics)
• Polymorphism, Single Nucleotide (genetics)
• RNA, Messenger (genetics, metabolism)
• Sus scrofa (genetics)
• Swine
• Swine Diseases (genetics, microbiology)